Anti-Raf1 (phospho Ser621) Anticorpo (A10884)

Name: Anti-Raf1 (phospho Ser621) Antibody
Brand: Antibodies.com
SKU: A10884
Price: 435.0 USD
Availability: InStock

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$435

Anticorpo policlonale di coniglio contro Raf1 (phospho Ser621) per WB e IP.

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Consegna

Spese di spedizione

$40

Tempi di consegna

5-8 Giorni lavorativi

Contattaci

Telefono

+1 (314) 370-6046

E-mail

orders@antibodies.com

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Descrizione Prodotto

Nome

Anti-Raf1 (phospho Ser621) Antibody

Descrizione

Rabbit polyclonal antibody to Raf1 (phospho Ser621).

Applicazioni

WB, IP

Diluizioni consigliate

WB: 1:500-1:2,000, IP: 1:50-1:100

Reattività

Human

Immunogeno

A synthetic phosphorylated peptide around S621 of human Phospho-Raf1-S621 (NP_002871.1).

Sequenza

SASEP

Specie ospite

Rabbit

Clonalità

Polyclonal

Isotipo

IgG

Coniugare

Unconjugated

Purificazione

Affinity purification.

Peso molecolare

73 kDa

Forma del prodotto

Liquid

Formulazione

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Sinonimi

c Raf, C-raf, C-Raf proto-oncogene, serine/threonine kinase, CMD1NN, cRaf, Craf 1 transforming gene, Craf1 transforming gene, EC 2.7.11.1, kinase Raf1, Murine sarcoma 3611 oncogene 1, NS5, Oncogene MIL, Oncogene RAF1, OTTHUMP00000160218, OTTHUMP00000207813, OTTHUMP00000209389, Protein kinase raf 1, Proto-oncogene c-RAF, RAF, Raf 1, Raf 1 proto oncogene serine/threonine kinase, Raf proto oncogene serine/threonine protein kinase, RAF proto-oncogene serine/threonine-protein kinase, RAF-1, RAF1_HUMAN, Similar to murine leukemia viral (V-raf-1) oncogene homolog 1, TRANSFORMING REPLICATION-DEFECTIVE MURINE RETROVIRUS 3611-MSV, v raf 1 murine leukemia viral oncogene homolog 1, v-raf murine sarcoma viral oncogene homolog 1, v-raf-1 murine leukemia viral oncogene-like protein 1, vraf1 murine leukemia viral oncogene homolog 1

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Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (2)

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Figure 1: Western Blot - Anti-Raf1 (phospho Ser621) Antibody (A10884)

Western blot analysis of extracts of HeLa and 293T cells, using Anti-Raf1 (phospho Ser621) Antibody (A10884) at 1:1,000 dilution. HeLa cells were treated by EGF (100ng/ml) for 30 minutes after serum-starvation overnight. 293T cells were treated by PMA/TPA (200nM) for 30 minutes or treated by EGF (25ug/mL) for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA.

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Figure 2: Western Blot - Anti-Raf1 (phospho Ser621) Antibody (A10884)

Immunoprecipitation analysis of 200µg extracts of HeLa cells using 3µg of Anti-Raf1 (phospho Ser621) Antibody (A10884). This Western blot was performed on the immunoprecipitate using Anti-Raf1 (phospho Ser621) Antibody (A10884) at a dilution of 1:1000. HeLa cells were treated by EGF (100 ng/ml) at 37°C for 30 minutes after serum-starvation overnight.