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Anti-Raf1 (phospho Ser338) Antibody (A10978)

Western Blot - Anti-Raf1 (phospho Ser338) Antibody (A10978) - Antibodies.com
$485
Product Datasheet

Rabbit polyclonal antibody to Raf1 (phospho Ser338) for WB.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Anti-Raf1 (phospho Ser338) Antibody
Description
Rabbit polyclonal antibody to Raf1 (phospho Ser338).
Applications
WB
Dilutions
WB: 1:500-1:2,000
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic phosphorylated peptide around S338 of human Raf1 (NP_002871.1).
Sequence
RDSSY
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
Lot Specific
Molecular Weight
73 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
cRaf, Proto-oncogene c-RAF, RAF, RAF proto-oncogene serine/threonine-protein kinase, Raf-1
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (1)

Western Blot - Anti-Raf1 (phospho Ser338) Antibody (A10978) - Antibodies.comEnlarge Image

Western Blot - Anti-Raf1 (phospho Ser338) Antibody (A10978)

Western blot analysis of extracts of NIH/3T3 cells, using Anti-Raf1 (phospho Ser338) Antibody (A10978) at 1:1,000 dilution or Raf1 antibody (A0223). NIH/3T3 cells were treated by PMA/TPA (200nM) for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 5s.
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