Contattaci
+1 (314) 370-6046 oContattaci
Visit our English Website
Antibodies.com LogoAntibodies.com Logo
Accedi / RegistratiIl Mio Account
0

Anti-Rad52 Anticorpo (A101677)

Nome
Anti-Rad52 Antibody
Descrizione
Rabbit polyclonal antibody to Rad52.
Applicazioni
WB, IP, IF, ELISA
Diluizioni consigliate
WB: 1:1,000-1:3,000, IP: 1:50-1:1,000, IF: 1:1,000
Reattività
Human, Mouse, Rat, Hamster
Immunogeno
Recombinant full length human Rad52 protein.
Specie ospite
Rabbit
Clonalità
Polyclonal
Isotipo
IgG
Coniugare

Unconjugated

Purificazione
Affinity purification.
Concentrazione
1 mg/ml
Forma del prodotto
Liquid
Formulazione
Supplied in 20mM Phosphate Buffered Saline, pH 7.5, with 0.15M Sodium Chloride and 50% Glycerol.
Conservazione
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Sinonimi
DNA repair protein RAD52, DNA repair protein RAD52 homolog, RAD 52, RAD52 homolog, RAD52 homolog (S. cerevisiae), RAD52_HUMAN, Recombination protein RAD52, Rhabdomyosarcoma antigen MU RMS 40.23
Controlli isotipo
Secondari adatti
Dichiarazione di non responsabilità
Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.
Western Blot of Rad52 in mammalian cells. M: Marker proteins. Lane 1: Recombinant human Rad52 protein (0.2ng). Lane 2: HeLa cell extrat (17µg). Lane 3: MCF7 cell extract (27µg). Lane 4: NIH3T3 cell extract (20µg). Lane 5: CHO cell extract (21µg). The samples were separated by electrophoresis on 10% SDS-PAGE and blotted onto PVDF membrane. Anti-Rad52 Antibody was used at 1:1,000 dilution and Goat Anti-Rabbit IgG Antibody (HRP) was used as a secondary at 1:10,000 dilution. Molecular mass of human Rad52 protein is 47 kDa.Clicca sull'immagine per la visualizzazione estesa
Figure 1: SDS-PAGE - Anti-Rad52 Antibody (70-015)
Anti-Rad52 Antibody
Western blot of Rad52 in Rat cells. Lane 1: Human MCF-7 cell lysate (16µg). Lane 2: Rat PC-12 cell (40µg). Proteins in the lysates were separated on SDS-PAGE (12.5%) and electro-blotted to PVDF membrane overnight. The blot was blocked with 5% skim milk. Anti-Rad52 Antibody was used at 1:1,000 dilution and Goat Anti-Rabbit IgG Antibody (HRP) was used as a secondary at 1:10,000 dilution.Clicca sull'immagine per la visualizzazione estesa
Figure 2: SDS-PAGE - Anti-Rad52 Antibody (70-015)
Anti-Rad52 Antibody
Immunoprecipitation of Rad52 from human cell lysate. 1. Add 10µg of Anti-Rad52 Antibody and 50µg of Dynabeads protein A. 2. Incubate for 10 min at room temperature, wash and resuspend in 100µl of the same buffer. 3. Add 100µl (300µg in PBS) lysate of human MCF7 cells. 4. Incubate for and collect the immunocomplex with Dynabeeds magnetic apparatus. 5. Elute the immunocomplex and analyze the presence of Rad52 in the supernatant (Lane 2) and the eluate (Lane 1) by western blotting with Anti-Rad52 Antibody. 6. Goat Anti-Rabbit IgG Antibody (HRP) was used as a secondary at 1:10,000 dilution. Lane 3 is western blot of MCF’ lysate (16µg).Clicca sull'immagine per la visualizzazione estesa
Figure 3: Immunoprecipitation - Anti-Rad52 Antibody (70-015)
Anti-Rad52 Antibody
HU-induced focus formation of Rad52. U2OS 40-2-6 cells were untreated or treated with 2mM hydroxyurea (HU) for 24 h. The cells were pre-extracted with 0.1% TritonX-100 in PBS, fixed with 3.7% formaldehyde in PBS, and immunostained with Anti-Rad52 Antibody (green) at 1:50 dilution, followed by DAPI staining (blue). Goat Anti-Rabbit IgG Antibody (CF488A) was used as a secondary at 1:1,000 dilution. TritonX-100 treatment extracts chromatin-free Rad52 from nuclei. Scale bars, 20 µm.Clicca sull'immagine per la visualizzazione estesa
Figure 4: Anti-Rad52 Antibody (70-015)
Anti-Rad52 Antibody

Prodotti alternativi a Anti-Rad52 Anticorpo (A101677)

Immunohistochemistry - Anti-RAD52 (phospho Tyr104) Antibody (A1119) - Antibodies.com
(2)
A93821
Anti-RAD52 (phospho Tyr104) Anticorpo
Anticorpo policlonale di coniglio contro RAD52 per WB, IHC e ELISA.
Segnaposto immagine Antibodies.com - Scopri di più spendendo meno
A96038
Anti-RAD52 Anticorpo
Anticorpo policlonale di coniglio contro RAD52 per WB, IHC, IF e ELISA.
Western Blot - Anti-RAD52 Antibody (A14337) - Antibodies.com
A14337
Anti-RAD52 Anticorpo
Anticorpo policlonale di coniglio contro RAD52 per WB.
Western Blot - Anti-RAD52 Antibody [ARC1183] (A308307) - Antibodies.com
A308307
Anti-RAD52 Anticorpo [ARC1183]
Anticorpo monoclonale [ARC1183] di coniglio contro RAD52 per WB.
Anti-Rad52 (N99) Antibody from Bioworld Technology (BS1691) - Antibodies.com
(2)
A25712
Anti-Rad52 (N99) Anticorpo
Anticorpo policlonale di coniglio contro Rad52 per WB, IHC e IF.
Western blot - RAD52 antibody from Signalway Antibody (38568) - Antibodies.com
(2)
A38144
Anti-RAD52 Anticorpo
Anticorpo policlonale di coniglio contro RAD52 per WB e IHC.
Anti-Rad52 (phospho-Y104) Antibody from Bioworld Technology (BS4869) - Antibodies.com
A27784
Anti-Rad52 (phospho-Y104) Anticorpo
Anticorpo policlonale di coniglio contro Rad52 per WB e IHC.
RAD52 Antibody from Signalway Antibody (37023) - Antibodies.com
(2)
A37991
Anti-RAD52 Anticorpo
Anticorpo policlonale di coniglio contro RAD52 per WB e IHC.
Immunohistochemistry - Anti-RAD52 (phospho Tyr104) Antibody (A1119) - Antibodies.com
(2)
A93821
Anti-RAD52 (phospho Tyr104) Anticorpo
Anticorpo policlonale di coniglio contro RAD52 per WB, IHC e ELISA.
Segnaposto immagine Antibodies.com - Scopri di più spendendo meno
A96038
Anti-RAD52 Anticorpo
Anticorpo policlonale di coniglio contro RAD52 per WB, IHC, IF e ELISA.
Western Blot - Anti-RAD52 Antibody (A14337) - Antibodies.com
A14337
Anti-RAD52 Anticorpo
Anticorpo policlonale di coniglio contro RAD52 per WB.
Western Blot - Anti-RAD52 Antibody [ARC1183] (A308307) - Antibodies.com
A308307
Anti-RAD52 Anticorpo [ARC1183]
Anticorpo monoclonale [ARC1183] di coniglio contro RAD52 per WB.
Anti-Rad52 (N99) Antibody from Bioworld Technology (BS1691) - Antibodies.com
(2)
A25712
Anti-Rad52 (N99) Anticorpo
Anticorpo policlonale di coniglio contro Rad52 per WB, IHC e IF.
Western blot - RAD52 antibody from Signalway Antibody (38568) - Antibodies.com
(2)
A38144
Anti-RAD52 Anticorpo
Anticorpo policlonale di coniglio contro RAD52 per WB e IHC.
Anti-Rad52 (phospho-Y104) Antibody from Bioworld Technology (BS4869) - Antibodies.com
A27784
Anti-Rad52 (phospho-Y104) Anticorpo
Anticorpo policlonale di coniglio contro Rad52 per WB e IHC.
RAD52 Antibody from Signalway Antibody (37023) - Antibodies.com
(2)
A37991
Anti-RAD52 Anticorpo
Anticorpo policlonale di coniglio contro RAD52 per WB e IHC.
Vedi tutto Anti-Rad52 Anticorpi
Menù Principale Contattaci 0Check-out
Inizio pagina