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Anti-Rad51 Anticorpo (A351)

Western blotting of endogenous Rad51 proteins in crude extracts of varius animal cells. 15% SDS-PAGE was used. Blotting was done at 15 v overnight with wet system. Anti-Rad51 Antibody was used at 1:1,000 dilution. Lane 1: MCF7 (human) cells (40µg). Lane 2: NIH3T3 (mouse) cells (40µg). Lane 3: CHO (hamster) cells (40µg). Lane 4: Xenopus laevis (frog) egg (40µg). Immunoprecipitation of Rad51 protein from crude extract of HeLa cells using Anti-Rad51 Antibody. Anti-Rad51 Antibody (20µg) was incubated with 20µg of HeLa cell extract, and precipitated with 20µg of protein A-beads. The sample was dissociated from the precipitate by heating in SDS-sample buffer and analyzed by western blotting with Anti-Rad51 Antibody (70-005) at 1:1,000 dilution. Rabbit Anti-Chicken IgG Antibody was used as a secondary at 1:10,000 dilution. Detection of Rad51 foci formation after X-ray irradiation in human fibroblast cell line, GM0637. Cells were irradiated by X-rays at 2 Gy, grown for 1 hr, fixed with 4% paraformaldehyde in 1x PBS for 10 min, washed 3 times with PBS for 3 min, permealized by treatment with 0.5% Triton for 5 min, washed 3 times with PBS for 3 min, incubated with Anti-Rad51 Antibody for 30 min at 37°C, washed 3 times with PBS for 3 min, incubated with secondary antibody for 30 min at 37°C, washed 3 times with PBS for 3 min, stained with Hoechst for 1 min and mounted. Anti-Rad51 Antibody was used at 1:10,000 dilution (left panels) and 1:1,000 dilution (right panels). Anti-Rabbit IgG Antibody (Alexa 488) was used as a secondary at 1:10,000 dilution. Immunohistological staining of Rad51 protein in mouse testis using Anti-Rad51 Antibody. A section of formalin fixed and paraffin embedded mouse testis was treated with the Anti-Rad51 Antibody at 1:100 dilution after deparaffization and antigen retrieval. Anti-Rabbit IgG Antibody (Alexa Fluor 647) was used at 1:1,000 dilution. DNA was stained with DAPI and the merged image was shown (Merged). The plain black and white microsopic picture of the same region was shown on the right. Western blot of endogenous Rad52 protein in rat cell lysate. Lane 1: Human MCF7 cell lysate (80µg). Lane 2: Rat PC12 cell lysate (80µg). Proteins in the lysates were separated on SDS-PAGE (12.5 % gel), and blotted to PVDF membrane at 15 V overnight. The membrane was blocked with 3% skim milk. Anti-Rad51 Antibody was used at 1:1,000 dilution and Goat Anti-Rabbit IgG Antibody was used as the secondary at 1:10,000. dilution.
$535

Anticorpo policlonale di coniglio contro Rad51 per WB, IP, ChIP, IF, IHC, DB e ELISA.

Garanzia al 100%
Garanzia di corrispondenza del prezzo

Spese di spedizione
$40
Tempi di consegna
4-6 Giorni lavorativi

Telefono
+1 (314) 370-6046
Lun - Ven, 8:00 - 16:00 AST
E-mail
orders@antibodies.com

Nome
Anti-Rad51 Antibody
Descrizione
Rabbit polyclonal antibody to Rad51.
Applicazioni
WB, IP, ChIP, IF, IHC, DB, ELISA
Diluizioni consigliate
WB: 1:1,000-1:10,000, IP: 1:1,00-1:1,000, IF: 1:1,000-1:10,000, IHC: 1:1,000-1:5,000, DB: 1:1,000-1:5,000, ELISA: 1:2,000-1:5,000
Reattività
Human, Mouse, Rat, Chicken, Xenopus
Immunogeno
Recombinant full length human Rad51 protein.
Specie ospite
Rabbit
Clonalità
Polyclonal
Isotipo
IgG
Coniugare

Unconjugated

Purificazione
Affinity purification.
Concentrazione
1 mg/ml
Forma del prodotto
Liquid
Formulazione
Supplied in Phosphate Buffered Saline with 50% Glycerol (filter sterilised, without Sodium Azide, and carrier free).
Conservazione
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Sinonimi
BRCA1/BRCA2 containing complex, subunit 5, BRCC 5, BRCC5, DNA repair protein RAD51 homolog 1, DNA repair protein rhp51, FANCR, hRAD51, HsRAD51, HsT16930, MRMV2, Rad 51, RAD51 homolog, RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae), RAD51 homolog A, RAD51 recombinase, RAD51, S. cerevisiae, homolog of, RAD51A, RAD51_HUMAN, RECA, RecA like protein, RecA, E. coli, homolog of, Recombination protein A
Controlli isotipo
Secondari adatti
Dichiarazione di non responsabilità
Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.
Western blotting of endogenous Rad51 proteins in crude extracts of varius animal cells. 15% SDS-PAGE was used. Blotting was done at 15 v overnight with wet system. Anti-Rad51 Antibody was used at 1:1,000 dilution. Lane 1: MCF7 (human) cells (40µg). Lane 2: NIH3T3 (mouse) cells (40µg). Lane 3: CHO (hamster) cells (40µg). Lane 4: Xenopus laevis (frog) egg (40µg).Clicca sull'immagine per la visualizzazione estesa
Figure 1: SDS-PAGE - Anti-Rad51 Antibody (70-012)
Anti-Rad51 Antibody
Immunoprecipitation of Rad51 protein from crude extract of HeLa cells using Anti-Rad51 Antibody. Anti-Rad51 Antibody (20µg) was incubated with 20µg of HeLa cell extract, and precipitated with 20µg of protein A-beads. The sample was dissociated from the precipitate by heating in SDS-sample buffer and analyzed by western blotting with Anti-Rad51 Antibody (70-005) at 1:1,000 dilution. Rabbit Anti-Chicken IgG Antibody was used as a secondary at 1:10,000 dilution.Clicca sull'immagine per la visualizzazione estesa
Figure 2: Immunoprecipitation - Anti-Rad51 Antibody (70-012)
Anti-Rad51 Antibody
Detection of Rad51 foci formation after X-ray irradiation in human fibroblast cell line, GM0637. Cells were irradiated by X-rays at 2 Gy, grown for 1 hr, fixed with 4% paraformaldehyde in 1x PBS for 10 min, washed 3 times with PBS for 3 min, permealized by treatment with 0.5% Triton for 5 min, washed 3 times with PBS for 3 min, incubated with Anti-Rad51 Antibody for 30 min at 37°C, washed 3 times with PBS for 3 min, incubated with secondary antibody for 30 min at 37°C, washed 3 times with PBS for 3 min, stained with Hoechst for 1 min and mounted. Anti-Rad51 Antibody was used at 1:10,000 dilution (left panels) and 1:1,000 dilution (right panels). Anti-Rabbit IgG Antibody (Alexa 488) was used as a secondary at 1:10,000 dilution.Clicca sull'immagine per la visualizzazione estesa
Figure 3: Anti-Rad51 Antibody (70-012)
Anti-Rad51 Antibody
Immunohistological staining of Rad51 protein in mouse testis using Anti-Rad51 Antibody. A section of formalin fixed and paraffin embedded mouse testis was treated with the Anti-Rad51 Antibody at 1:100 dilution after deparaffization and antigen retrieval. Anti-Rabbit IgG Antibody (Alexa Fluor 647) was used at 1:1,000 dilution. DNA was stained with DAPI and the merged image was shown (Merged). The plain black and white microsopic picture of the same region was shown on the right.Clicca sull'immagine per la visualizzazione estesa
Figure 4: Anti-Rad51 Antibody (70-012)
Anti-Rad51 Antibody

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