Anti-Rad21 Anticorpo (A305614)

Name: Anti-Rad21 Antibody
Brand: Antibodies.com
SKU: A305614
Price: 335.0 USD
Availability: InStock

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$335

Anticorpo policlonale di coniglio contro Rad21 per WB, IHC, ICC/IF, IP e ChIP.

Garanzia al 100%

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Consegna

Spese di spedizione

$40

Tempi di consegna

5-8 Giorni lavorativi

Contattaci

Telefono

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanzie

Garanzia di qualità al 100%

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Descrizione Prodotto

Nome

Anti-Rad21 Antibody

Descrizione

Rabbit polyclonal antibody to Rad21.

Applicazioni

WB, IHC, ICC/IF, IP, ChIP

Diluizioni consigliate

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000, ChIP: 1:500-1:1,000

Reattività

Human, Mouse, Rat

Immunogeno

Recombinant fusion protein containing a sequence corresponding to amino acids 268-429 of human Rad21 (NP_006256.1).

Sequenza

DNVSMGGPDSPDSVDPVEPMPTMTDQTTLVPNEEEAFALEPIDITVKETKAKRKRKLIVDSVKELDSKTIRAQLSDYSDIVTTLDLAPPTKKLMMWKETGGVEKLFSLPAQPLWNNRLLKLFTRCLTPLVPEDLRKRRKGGEADNLDEFLKEFENPEVPRED

Specie ospite

Rabbit

Clonalità

Polyclonal

Isotipo

IgG

Coniugare

Unconjugated

Purificazione

Affinity purification.

Peso molecolare

130 kDa

Forma del prodotto

Liquid

Formulazione

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Sinonimi

CDLS4, Double-strand-break repair protein rad21 homolog, hHR21, HR21, HRAD21, KIAA0078, MCD1, Nuclear matrix protein 1, NXP-1, NXP1, Protein involved in DNA double-strand break repair, RAD21 homolog, RAD21 homolog (S. pombe), RAD21_HUMAN, Scc1, SCC1 homolog

Controlli isotipo

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (10)

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Figure 1: Western Blot - Anti-Rad21 Antibody (A305614)

Western blot analysis of extracts of various cell lines, using Anti-Rad21 Antibody (A305614) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 2: Western Blot - Anti-Rad21 Antibody (A305614)

Western blot analysis of extracts of C6 cells, using Anti-Rad21 Antibody (A305614) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 90s.

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Figure 3: Immunohistochemistry - Anti-Rad21 Antibody (A305614)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-Rad21 Antibody (A305614) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-Rad21 Antibody (A305614)

Immunohistochemistry analysis of paraffin-embedded mouse stomach using Anti-Rad21 Antibody (A305614) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 5: Immunohistochemistry - Anti-Rad21 Antibody (A305614)

Immunohistochemistry analysis of paraffin-embedded rat stomach using Anti-Rad21 Antibody (A305614) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 6: Immunofluorescence - Anti-Rad21 Antibody (A305614)

Immunofluorescence analysis of U2OS cells using Anti-Rad21 Antibody (A305614) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 7: Western Blot - Anti-Rad21 Antibody (A305614)

Immunoprecipitation analysis of 300µg extracts of Jurkat cells using 3µg of Anti-Rad21 Antibody (A305614). This Western blot was performed on the immunoprecipitate using Anti-Rad21 Antibody (A305614) at a dilution of 1:1000.

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Figure 8: Western Blot - Anti-Rad21 Antibody (A305614)

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Figure 9: Chromatin Immunoprecipitation - Anti-Rad21 Antibody (A305614)

Chromatin immunoprecipitation (ChIP) analysis of extracts of A-549 cells, using Anti-Rad21 Antibody (A305614) and Rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.