Anti-RAD18 Antibody (A91251)

Name: Anti-RAD18 Antibody
Brand: Antibodies.com
SKU: A91251
Price: 355.0 USD
Availability: InStock

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$355

Rabbit polyclonal antibody to RAD18 for WB, IHC and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-RAD18 Antibody

Description

Rabbit polyclonal antibody to RAD18.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 216-495 of human Rad18 (NP_064550.3).

Sequence

INKHLDSCLSREEKKESLRSSVHKRKPLPKTVYNLLSDRDLKKKLKEHGLSIQGNKQQLIKRHQEFVHMYNAQCDALHPKSAAEIVREIENIEKTRMRLEASKLNESVMVFTKDQTEKEIDEIHSKYRKKHKSEFQLLVDQARKGYKKIAGMSQKTVTITKEDESTEKLSSVCMGQEDNMTSVTNHFSQSKLDSPEELEPDREEDSSSCIDIQEVLSSSESDSCNSSSSDIIRDLLEEEEAWEASHKNDLQDTEISPRQNRRTRAAESAEIEPRNKRNRN

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

80 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

E3 ubiquitin-protein ligase RAD18, hHR18, hRAD18, Postreplication repair protein RAD18, RING finger protein 73, RING-type E3 ubiquitin transferase RAD18, RNF73

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-RAD18 Antibody (A91251)

Western blot analysis of extracts of various cell lines, using Anti-RAD18 Antibody (A91251) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 120s.

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Immunofluorescence - Anti-RAD18 Antibody (A91251)

Immunofluorescence analysis of U2OS cells using Anti-RAD18 Antibody (A91251). DAPI was used to stain the cell nuclei (blue).

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Immunofluorescence - Anti-RAD18 Antibody (A91251)

Immunofluorescence analysis of U2OS cells using Anti-RAD18 Antibody (A91251). DAPI was used to stain the cell nuclei (blue). DNA damage by a UV-A laser.

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