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Anti-Rad18 Anticorpo (A354)

Identification of mouse Rad18 protein in ES cells by western blotting with Anti-Rad18 Antibody. WT: Lysate of wild-type mouse ES cells RAD18-/- ; Lysate of Rad18 double knock-out mouse ES cells. Protein levels of alpha Tubulin in the lysates are shown as a control. Three bands are absent in RAD18 knock-out cells. Identification of mouse Rad18 protein in NIH3T3 cells by western blotting with Anti-Rad18 Antibody. Cell extract, 23µg, was used. Anti-Rad18 Antibody was used at 1:2,000 dilution. Two extra bands (75 + 90 kDa) may represent modified products. Immunofluorescence staining of Rad18 protein with Anti-Rad18 Antibody. Wild-type (+/+) and RAD18-/- ES cells (-/-). Samples were prefixed 3.7% formaldehyde and fixed with 80% methanol. Anti-Rad18 Antibody was used at 1:300 dilution. Anti-Rabbit IgG (FITC) was used as a secondary antibody. Detection of Rad18 in mouse testis. Section of paraformaldehyde fixed mouse was stained with Anti-Rad18 Antibody. Donkey Anti-Rabbit IgG Antibody (HRP) was used as a secondary antibody. Signals were enhanced with TSA plus biotin system and detected by using DAB substrate. Arrows indicate undifferentiated spermatogonia.
Knockout Convalidato
$535

Anticorpo policlonale di coniglio contro Rad18 per WB, IP, IF e IHC.

Garanzia al 100%
Garanzia di corrispondenza del prezzo

Spese di spedizione
$40
Tempi di consegna
4-6 Giorni lavorativi

Telefono
+1 (314) 370-6046
Lun - Ven, 8:00 - 16:00 AST
E-mail
orders@antibodies.com

Nome
Anti-Rad18 Antibody
Descrizione
Rabbit polyclonal antibody to Rad18.
Applicazioni
WB, IP, IF, IHC
Diluizioni consigliate
WB: 1:1,000, IP: 1:200-1:500, IHC: 1:100-1:300
Reattività
Mouse
Immunogeno
Synthetic peptide containing 100 carboxyl terminal amino acids of mouse Rad18 protein (GST tag).
Specie ospite
Rabbit
Clonalità
Polyclonal
Isotipo
IgG
Coniugare

Unconjugated

Purificazione
Affinity purification.
Concentrazione
1 mg/ml
Forma del prodotto
Liquid
Formulazione
Supplied in Phosphate Buffered Saline with 50% Glycerol (filter sterilised, without Sodium Azide, and carrier free).
Conservazione
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Sinonimi
2810024C04Rik, DNA repair protein rad18, E3 ubiquitin-protein ligase RAD18, EC 6.3.2.-, hHR 18, hHR18, hRAD 18, hRAD18, MGC156682, Postreplication repair E3 ubiquitin-protein ligase RAD18, Postreplication repair protein hRAD18p, Postreplication repair protein RAD18, RAD 18, RAD18 homolog, RAD18 homolog (S. cerevisiae), RAD18 S. cerevisiae homolog, RAD18 S. cerevisiae homolog of, RAD18 transcript variant, Rad18sc, RAD18_HUMAN, Radiation sensitivity protein 18, RING finger protein 73, RNF 73, RNF73, Structural maintenance of chromosomes protein 6, YCR066W, YCR66W
Controlli isotipo
Secondari adatti
Dichiarazione di non responsabilità
Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.
Identification of mouse Rad18 protein in ES cells by western blotting with Anti-Rad18 Antibody. WT: Lysate of wild-type mouse ES cells RAD18-/- ; Lysate of Rad18 double knock-out mouse ES cells. Protein levels of alpha Tubulin in the lysates are shown as a control. Three bands are absent in RAD18 knock-out cells.Clicca sull'immagine per la visualizzazione estesa
Figure 1: Anti-Rad18 Antibody (70-025)
Anti-Rad18 Antibody
Identification of mouse Rad18 protein in NIH3T3 cells by western blotting with Anti-Rad18 Antibody. Cell extract, 23µg, was used. Anti-Rad18 Antibody was used at 1:2,000 dilution. Two extra bands (75 + 90 kDa) may represent modified products.Clicca sull'immagine per la visualizzazione estesa
Figure 2: Anti-Rad18 Antibody (70-025)
Anti-Rad18 Antibody
Immunofluorescence staining of Rad18 protein with Anti-Rad18 Antibody. Wild-type (+/+) and RAD18-/- ES cells (-/-). Samples were prefixed 3.7% formaldehyde and fixed with 80% methanol. Anti-Rad18 Antibody was used at 1:300 dilution. Anti-Rabbit IgG (FITC) was used as a secondary antibody.Clicca sull'immagine per la visualizzazione estesa
Figure 3: Immunofluorescence - Anti-Rad18 Antibody (70-025)
Anti-Rad18 Antibody
Detection of Rad18 in mouse testis. Section of paraformaldehyde fixed mouse was stained with Anti-Rad18 Antibody. Donkey Anti-Rabbit IgG Antibody (HRP) was used as a secondary antibody. Signals were enhanced with TSA plus biotin system and detected by using DAB substrate. Arrows indicate undifferentiated spermatogonia.Clicca sull'immagine per la visualizzazione estesa
Figure 4: Anti-Rad18 Antibody (70-025)
Anti-Rad18 Antibody

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