Anti-QKI Anticorpo [PCRP-QKI-2F10] (A278440)

Name: Anti-QKI Antibody [PCRP-QKI-2F10] - BSA and Azide free
Brand: Antibodies.com
SKU: A278440
Price: 525.0 USD
Availability: InStock

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$525

Anticorpo monoclonale [PCRP-QKI-2F10] di topo contro QKI per ELISA, Flow Cytometry, IP, IF e WB.

Garanzia al 100%

Garanzia di corrispondenza del prezzo

Alternative Formats and ConjugatesAlternative Formats

Unconjugated

Consegna

Spese di spedizione

$40

Tempi di consegna

4-6 Giorni lavorativi

Contattaci

Telefono

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanzie

Garanzia di qualità al 100%

Garanzia di corrispondenza del prezzo

Tag dei prodotti:

Anticorpo Monospecifico

Descrizione Prodotto

Nome

Anti-QKI Antibody [PCRP-QKI-2F10] - BSA and Azide free

Descrizione

Mouse monoclonal [PCRP-QKI-2F10] antibody to QKI.

Specificità

QKI, also known as HKQ, QK, QK3 or quaking, is a 341 amino acid protein that localizes to both the cytoplasm and the nucleus and contains one KH domain. Expressed in the frontal cortex of the brain, QKI functions as an RNA-binding protein that plays an important role in myelinization and specifically binds to the RNA core sequence 5'-NACUAAY-N(1,20)-UAAY-3'. Additionally, QKI regulates pre-mRNA splicing, and mRNA export and is involved in protecting and promoting the stability of select mRNAs. QKI may be methylated by PRMT1 and may also be phosphorylated at its C-terminus, an event that decreases QKI mRNA-binding affinity. Defects or deletions in the gene encoding QKI are associated with astrocytic tumors and may be involved in the pathogenesis of schizophrenia. Multiple isoforms of QKI exist due to alternative splicing events.

Applicazioni

ELISA, Flow Cytometry, IP, IF, WB

Diluizioni consigliate

Flow Cytometry: 1-2 µg/million cells, IP: 1-2µg / 100-500µg proteins, IF: 1-2 µg/ml, WB: 1-2 µg/ml

Reattività

Human

Immunogeno

Recombinant full-length human QKI protein.

Specie ospite

Mouse

Clonalità

Monoclonal

Clona ID

PCRP-QKI-2F10

Isotipo

IgG2c

Coniugare

Unconjugated

Purificazione

Protein A/G chromatography.

Concentrazione

1 mg/ml

Peso molecolare

37.6 kDa

Forma del prodotto

Liquid

Formulazione

Supplied in 10mM Phosphate Buffered Saline; without Sodium Azide and carrier free.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Note generali

This monoclonal antibody is also available in a different formulation with BSA and Sodium Azide - Anti-QKI Antibody [PCRP-QKI-2F10] (A277852).

Sinonimi

DKFZp586I0923, HKQ, Homolog of mouse quaking QKI KH domain RNA binding protein, Hqk, HQK1, HqkI, OTTHUMP00000017581, OTTHUMP00000017582, OTTHUMP00000017583, Protein quaking, QK, QK1, QK3, QKI1, QKI_HUMAN, Quaking homolog, Quaking homolog KH domain RNA binding, Quaking homolog KH domain RNA binding mouse, Quaking isoform 1, Quaking protein, RNA binding protein HQK

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (9)

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Figure 1: SDS-PAGE - Anti-QKI Antibody [PCRP-QKI-2F10] - BSA and Azide free (A278440)

SDS-PAGE analysis of Anti-QKI Antibody [PCRP-QKI-2F10] under non-reduced and reduced conditions; showing intact IgG and intact heavy and light chains, respectively. SDS-PAGE analysis confirms the integrity and purity of the antibody.

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Figure 2: Flow Cytometry - Anti-QKI Antibody [PCRP-QKI-2F10] - BSA and Azide free (A278440)

Flow cytometric analysis of PFA-fixed HeLa cells using Anti-QKI Antibody [PCRP-QKI-2F10] followed by Goat Anti-Mouse IgG (CF® 488) (Blue). Isotype Control (Red).

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Figure 3: Flow Cytometry - Anti-QKI Antibody [PCRP-QKI-2F10] - BSA and Azide free (A278440)

Flow cytometric analysis of PFA-fixed K562 cells using Anti-QKI Antibody [PCRP-QKI-2F10] followed by Goat Anti-Mouse IgG (CF® 488) (Blue). Isotype Control (Red).

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Figure 4: Flow Cytometry - Anti-QKI Antibody [PCRP-QKI-2F10] - BSA and Azide free (A278440)

Flow cytometric analysis of PFA-fixed U87 cells using Anti-QKI Antibody [PCRP-QKI-2F10] followed by Goat Anti-Mouse IgG (CF® 488) (Blue). Isotype Control (Red).

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Figure 5: Flow Cytometry - Anti-QKI Antibody [PCRP-QKI-2F10] - BSA and Azide free (A278440)

Flow cytometric analysis of PFA-fixed MCF-7 cells using Anti-QKI Antibody [PCRP-QKI-2F10] followed by Goat Anti-Mouse IgG (CF® 488) (Blue). Isotype Control (Red).

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Figure 6: Immunofluorescence - Anti-QKI Antibody [PCRP-QKI-2F10] - BSA and Azide free (A278440)

Immunofluorescent analysis of PFA-fixed U87 cells stained with Anti-QKI Antibody [PCRP-QKI-2F10] followed by Goat Anti-Mouse IgG (CF® 488) (Green). CF® 640R Phalloidin (Red).

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Figure 7: Immunofluorescence - Anti-QKI Antibody [PCRP-QKI-2F10] - BSA and Azide free (A278440)

Immunofluorescent analysis of K562 cells stained with Anti-QKI Antibody [PCRP-QKI-2F10] followed by Goat Anti-Mouse IgG (CF® 488) (Green). CF® 640R Phalloidin (Red).

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Figure 8: Immunofluorescence - Anti-QKI Antibody [PCRP-QKI-2F10] - BSA and Azide free (A278440)

Immunofluorescent analysis of PFA-fixed MCF-7 cells stained with Anti-QKI Antibody [PCRP-QKI-2F10] followed by Goat Anti-Mouse IgG (CF® 488) (Green). CF® 640R Phalloidin (Red).

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Figure 9: Anti-QKI Antibody [PCRP-QKI-2F10] - BSA and Azide free (A278440)

Analysis of protein array containing more than 19,000 full-length human proteins using Anti-QKI Antibody [PCRP-QKI-2F10]. Z-Score and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt^TM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt^TM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target; a MAb is considered to be specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

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