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Anti-PURA Anticorpo (A390)

Identification of endogenous Pura protein in whole cell extracts of HeLa cells and MCF-7 cells. Arrow indicates the position of PURA bands The anti-Pura antibody was used at 1/1,000 dilution. 12.5% SDS-PAGE was used. Blotting was done in wet system at 15 v overnight. CanGetSignal (Toyobo, Osaka) was used as a signal enhancer Identification of Pura protein band by western blotting, using siRNA. HCT116 cells were transfected with siPura and the cell lysates were prepared after 48 h. The Pura band was indicated by an arrow at 38 kDa position. Immunoprecipitation of Pura protein from whole cell lysate of HeLa cells with anti-PURA antibody. Whole cell lysate of HeLa cells was reacted with anti-Pura antibody and precipitated with protein G conjugated magnetic beads, and analyzed by WB by using anti-Pura antibody. As the secondary antibody, anti-rabbit IgG antibody conjugated with HRP was used. Immunofluorescence attaining of Pura protein in HeLa cells with Anti-Pura antibody. The anti-Pura antibody was used at 1/100 dilution and as the second antibody, Alexa 555-conjugated goat anti-rabbit IgG antibody was used at 1/1,000 dilution. DNA was stained with DAPI.
$535

Anticorpo policlonale di coniglio contro PURA per WB, IP e IF.

Garanzia al 100%
Garanzia di corrispondenza del prezzo

Spese di spedizione
$40
Tempi di consegna
4-6 Giorni lavorativi

Telefono
+1 (314) 370-6046
Lun - Ven, 8:00 - 16:00 AST
E-mail
orders@antibodies.com

Nome
Anti-PURA Antibody
Descrizione
Rabbit polyclonal antibody to PURA.
Applicazioni
WB, IP, IF
Diluizioni consigliate
WB: 1:1,000-1:3,000, IP: 1:1,000, IF: 1:100-1:500
Reattività
Human, Mouse
Immunogeno
Recombinant full length human PURA protein (GST tag), expressed in E. coli.
Specie ospite
Rabbit
Clonalità
Polyclonal
Isotipo
IgG
Coniugare

Unconjugated

Purificazione
Affinity purification.
Concentrazione
1 mg/ml
Forma del prodotto
Liquid
Formulazione
Supplied in Phosphate Buffered Saline with 50% Glycerol (filter sterilised, without Sodium Azide, and carrier free).
Conservazione
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Sinonimi
PUR ALPHA, PUR1, PURALPHA, PURA_HUMAN, Purine rich element binding protein A, Purine rich single stranded DNA binding protein alpha, Purine-rich single-stranded DNA-binding protein alpha, Transcriptional activator protein PUR alpha, Transcriptional activator protein Pur-alpha
Controlli isotipo
Secondari adatti
Dichiarazione di non responsabilità
Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.
Identification of endogenous Pura protein in whole cell extracts of HeLa cells and MCF-7 cells. Arrow indicates the position of PURA bands The anti-Pura antibody was used at 1/1,000 dilution. 12.5% SDS-PAGE was used. Blotting was done in wet system at 15 v overnight. CanGetSignal (Toyobo, Osaka) was used as a signal enhancerClicca sull'immagine per la visualizzazione estesa
Figure 1: SDS-PAGE - Anti-PURA Antibody (70-460)
Anti-PURA Antibody
Identification of Pura protein band by western blotting, using siRNA. HCT116 cells were transfected with siPura and the cell lysates were prepared after 48 h. The Pura band was indicated by an arrow at 38 kDa position.Clicca sull'immagine per la visualizzazione estesa
Figure 2: Anti-PURA Antibody (70-460)
Anti-PURA Antibody
Immunoprecipitation of Pura protein from whole cell lysate of HeLa cells with anti-PURA antibody. Whole cell lysate of HeLa cells was reacted with anti-Pura antibody and precipitated with protein G conjugated magnetic beads, and analyzed by WB by using anti-Pura antibody. As the secondary antibody, anti-rabbit IgG antibody conjugated with HRP was used.Clicca sull'immagine per la visualizzazione estesa
Figure 3: Immunoprecipitation - Anti-PURA Antibody (70-460)
Anti-PURA Antibody
Immunofluorescence attaining of Pura protein in HeLa cells with Anti-Pura antibody. The anti-Pura antibody was used at 1/100 dilution and as the second antibody, Alexa 555-conjugated goat anti-rabbit IgG antibody was used at 1/1,000 dilution. DNA was stained with DAPI.Clicca sull'immagine per la visualizzazione estesa
Figure 4: Immunofluorescence - Anti-PURA Antibody (70-460)
Anti-PURA Antibody
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