Contattaci
+1 (314) 370-6046 oContattaci
Visit our English Website
Antibodies.com LogoAntibodies.com Logo
Accedi / RegistratiIl Mio Account
0

PSMD2 Cell Based Kit Elisa (A103160)

Nome
PSMD2 Cell Based ELISA Kit
Tipo di kit
Cell-based (qualitative)
Principio di kit
This PSMD2 Cell Based ELISA Kit allows for the detection of PSMD2 and the effects that certain stimulation conditions have on PSMD2 expression in different cell lines. Qualitative determination of PSMD2 concentration is achieved by an indirect ELISA format. In essence, the PSMD2 is captured by Anti-PSMD2 Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this PSMD2 Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.
Piattaforma
Microplate
Tipo di rilevamento
Colorimetric
Tipo di campione
Adherent cells and suspension cells.
Campo di rilevamento
> 5000 Cells
Tempo del kit
4 hours 30 minutes
Reattività
Human, Mouse, Rat
Precision
Intra-assay CV: < 8%, Inter-assay CV: < 10%
Conservazione
Store at + 4°C. Stable for 6 months.
Sinonimi
26S proteasome non-ATPase regulatory subunit 2, 26S proteasome regulatory subunit RPN1, 26S proteasome regulatory subunit S2, 26S proteasome subunit p97, 55.11 protein, MGC14274, P97, Proteasome (prosome macropain) 26S subunit non ATPase 2, Proteasome 26S S2, Proteasome 26S subunit, non-ATPase, 2, Proteasome 26S, subunit 2, Protein 55.11, PSMD2_HUMAN, Rpn1, S2, TNFR associated protein 2, TRAP2, Tumor necrosis factor receptor associated protein 2, Tumor necrosis factor type 1 receptor-associated protein 2
Dichiarazione di non responsabilità
Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.
Western Blot - PSMD2 Cell Based ELISA Kit (CB5981) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 1: Western Blot - PSMD2 Cell Based ELISA Kit (CB5981)
Western blot analysis of extracts from COLO205/293 cells, using Anti-PSMD2 Antibody. The lane on the right is treated with the synthesized peptide.
Protocol Diagram - PSMD2 Cell Based ELISA Kit (CB5981) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 2: PSMD2 Cell Based ELISA Kit (CB5981)
A diagram showing the protocol for PSMD2 Cell Based ELISA Kit.
Protocol Summary - PSMD2 Cell Based ELISA Kit (CB5981) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 3: PSMD2 Cell Based ELISA Kit (CB5981)
A summary of the protocol for PSMD2 Cell Based ELISA Kit.
Western Blot - Control Antibody - PSMD2 Cell Based ELISA Kit (CB5981) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 4: Western Blot - PSMD2 Cell Based ELISA Kit (CB5981)
The mouse monoclonal antibody to GAPDH is used as a positive control inPSMD2 Cell Based ELISA Kit. Anti-GAPDH Antibody was tested for specificity by western blot with tissue lysates from human, mouse, and rat.
Menù Principale Contattaci 0Check-out
Inizio pagina