Anti-PSMA Anticorpo [GCP-05] (A85667)

Name: Anti-PSMA Antibody [GCP-05]
Brand: Antibodies.com
SKU: A85667
Price: 195.0 USD
Availability: InStock

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$195

Anticorpo monoclonale [GCP-05] di topo contro PSMA per Flow Cytometry, IP, ICC e CyTOF.

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Consegna

Spese di spedizione

$40

Tempi di consegna

4-6 Giorni lavorativi

Contattaci

Telefono

+1 (314) 370-6046

E-mail

orders@antibodies.com

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Descrizione Prodotto

Nome

Anti-PSMA Antibody [GCP-05]

Descrizione

Mouse monoclonal [GCP-05] antibody to PSMA.

Specificità

This antibody recognizes extracellular domain (preferentially in native form) of glutamate carboxypeptidase II (NAALADase, FOLH1, PSMA), an approximately 95-110 kDa transmembrane glycoprotein expressed mainly in tumour neovasculatures, nervous system, and jejunum. It is an important prostate tumour marker.

Applicazioni

Flow Cytometry, IP, ICC, CyTOF

Diluizioni consigliate

Flow Cytometry: 1-4 µg/ml.

Reattività

Human

Immunogeno

Recombinant protein corresponding to amino acids 44-750 of human GCPII.

Specie ospite

Mouse

Clonalità

Monoclonal

Clona ID

GCP-05

Isotipo

IgG1

Coniugare

Unconjugated

Purificazione

Protein A chromatography.

Concentrazione

1 mg/ml

MW previsto

95-110 kDa

Forma del prodotto

Liquid

Formulazione

Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Sinonimi

Cell growth inhibiting protein 27, Cell growth-inhibiting gene 27 protein, FGCP, Folate hydrolase, Folate hydrolase (prostate-specific membrane antigen) 1, Folate hydrolase 1, Folate hydrolase prostate specific membrane antigen 1, FOLH, FOLH 1, Folh1, FOLH1_HUMAN, Folylpoly gamma glutamate carboxypeptidase, Folylpoly-gamma-glutamate carboxypeptidase, GCP 2, GCP II, GCP2, GCPII, GIG27, Glutamate carboxylase II, Glutamate carboxypeptidase 2, Glutamate carboxypeptidase II, Membrane glutamate carboxypeptidase, mGCP, N acetylated alpha linked acidic dipeptidase 1, N-acetylated-alpha-linked acidic dipeptidase I, NAALAD 1, NAALAD1, NAALAdase, NAALADase I, Prostate specific membrane antigen, Prostate specific membrane antigen variant F, Prostate-specific membrane antigen, PSM, Pteroylpoly gamma glutamate carboxypeptidase, Pteroylpoly-gamma-glutamate carboxypeptidase

Controlli isotipo

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (2)

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Figure 1: Flow Cytometry - Anti-PSMA Antibody [GCP-05] (A85667)

Surface staining (flow cytometry) of GCPII / PSMA using Anti-GCPII Antibody (A85667) and Goat Anti-Mouse IgG Antibody (PE) on LNCaP cell line (positive, green) and HeLa cells (negative, blue).

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Figure 2: Anti-PSMA Antibody [GCP-05] (A85667)

Surface staining (mass cytometry) of LNCaP cell line using Anti-GCPII Antibody (A85667). Gated on singlets.

Pubblicazioni per questo clone (1)

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(2)

Characterization of a novel metastatic prostate cancer cell line of LNCaP origin

Riferimenti: Anti-PSMA Antibody [GCP-05] (ab66912)

Astratto:

Background: The LNCaP cell line was originally isolated from the lymph node of a patient with metastatic prostate cancer. Many cell lines have been derived from LNCaP by selective pressures to study different aspects of prostate cancer progression. When injected subcutaneously into male athymic nude mice, LNCaP and its derivatives rarely metastasize.

Methods: Here, we describe the characteristics of a new LNCaP derivative, JHU-LNCaP-SM, which was generated by long term passage in normal cell culture conditions.

Results: Short tandem repeat (STR) analysis and genomic sequencing verified JHU-LNCaP-SM derivation from parental LNCaP cells. JHU-LNCaP-SM cells express the same mutated androgen receptor (AR) but unlike LNCaP, are no longer androgen dependent for growth. The cells demonstrate an attenuated androgen responsiveness in transcriptional assays and retain androgen sensitive expression of PSA, AR, and PSMA. Unlike parental LNCaP, JHU-LNCaP-SM cells quickly form subcutaneous tumors in male athymic nude mice, reliably metastasize to the lymph nodes and display a striking intra-tumoral and spreading hemorrhagic phenotype as tumor xenografts.

Conclusions: The JHU-LNCaP-SM cell line is a new isolate of LNCaP, which facilitates practical, preclinical studies of spontaneous metastasis of prostate cancer through lymphatic tissues.

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