Western blot analysis of extracts of various cell lines, using Anti-Prohibitin Antibody (A12508) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-Prohibitin Antibody (A12508) at a dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-Prohibitin Antibody (A12508) at a dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-Prohibitin Antibody (A12508) at a dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded rat kidney using Anti-Prohibitin Antibody (A12508) at a dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of L929 cells using Anti-Prohibitin Antibody (A12508) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
![Immunohistochemistry - Anti-Prohibitin Antibody [PHB/3225] (A249627) - Antibodies.com](https://cdn.antibodies.com/image/catalog/249/A249627_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-Prohibitin Antibody [PHB/3225] - BSA and Azide free (A252807) - Antibodies.com](https://cdn.antibodies.com/image/catalog/252/A252807_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-Prohibitin Antibody [PHB/3194] (A249626) - Antibodies.com](https://cdn.antibodies.com/image/catalog/249/A249626_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-Prohibitin Antibody [PHB/3194] - BSA and Azide free (A252806) - Antibodies.com](https://cdn.antibodies.com/image/catalog/252/A252806_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-Prohibitin Antibody [PHB/3231] (A249621) - Antibodies.com](https://cdn.antibodies.com/image/catalog/249/A249621_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-Prohibitin Antibody [PHB/3231] - BSA and Azide free (A252801) - Antibodies.com](https://cdn.antibodies.com/image/catalog/252/A252801_1.jpg?profile=product_alternative)
![Western Blot - Anti-Prohibitin Antibody [ARC0007] (A305437) - Antibodies.com](https://cdn.antibodies.com/image/catalog/305/A305437_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-Prohibitin Antibody [PHB/1882] (A249623) - Antibodies.com](https://cdn.antibodies.com/image/catalog/249/A249624_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-Prohibitin Antibody [PHB/1882] - BSA and Azide free (A252803) - Antibodies.com](https://cdn.antibodies.com/image/catalog/252/A252804_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-Prohibitin Antibody [PHB/3230] - BSA and Azide free (A252800) - Antibodies.com](https://cdn.antibodies.com/image/catalog/252/A252800_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-Prohibitin Antibody [PHB/3227] (A249629) - Antibodies.com](https://cdn.antibodies.com/image/catalog/249/A249629_1.jpg?profile=product_alternative)