Figure 1: Western Blot - Anti-PRAF1 Antibody (A90543)
Western blot analysis of extracts of various cell lines, using Anti-PRAF1 Antibody (A90543) at 1:3,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
Figure 2: Western Blot - Anti-PRAF1 Antibody (A90543)
Immunoprecipitation analysis of 200µg extracts of Jurkat cells using 3µg of Anti-PRAF1 Antibody (A90543). This Western blot was performed on the immunoprecipitate using Anti-PRAF1 Antibody (A90543) at a dilution of 1:1000.
