Anti-PNPLA3 Anticorpo (A82487)

Name: Anti-PNPLA3 Antibody
Brand: Antibodies.com
SKU: A82487
Price: 480.0 USD
Availability: InStock

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$480

Anticorpo policlonale di capra contro PNPLA3 per ELISA, WB e IHC.

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Consegna

Spese di spedizione

$40

Tempi di consegna

3-5 Giorni lavorativi

Contattaci

Telefono

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanzie

Garanzia di qualità al 100%

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Descrizione Prodotto

Nome

Anti-PNPLA3 Antibody

Descrizione

Goat polyclonal antibody to PNPLA3.

Applicazioni

ELISA, WB, IHC

Diluizioni consigliate

ELISA: 1:16,000, WB: 0.01-0.03µg/ml, IHC: 8µg/ml

Reattività

Human, Mouse

Immunogeno

Synthetic peptide corresponding to the internal region of mouse PNPLA3.

Sequenza

EHDICPKVKSTN

Specie ospite

Goat

Clonalità

Polyclonal

Isotipo

IgG

Coniugare

Unconjugated

Purificazione

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentrazione

500 µg/ml

Peso molecolare

45 kDa

MW previsto

42.6Da

Forma del prodotto

Liquid

Formulazione

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Sinonimi

Acylglycerol O acyltransferase, Adiponutrin, ADPN, C22orf20, Calcium independent phospholipase A2 epsilon, DJ796I17.1, iPLA(2)epsilon, IPLA2-epsilon, Patatin like phospholipase domain containing 3, patatin like phospholipase domain containing protein 3, phospholipase A2, calcium independent, epsilon

Controlli isotipo

Goat IgG (A121671)

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (4)

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Figure 1: Western Blot - Anti-PNPLA3 Antibody (A82487)

PNPLA3 expression in Mouse Adrenal Gland lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-PNPLA3 Antibody (A82487) at 0.03µg/ml and detected by chemiluminescence.

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Figure 2: Immunohistochemistry - Anti-PNPLA3 Antibody (A82487)

PNPLA3 expression in Human Kidney analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by heating in citrate buffer, pH 6. Staining was performed with Anti-PNPLA3 Antibody (A82487) at 8µg/ml and revealed with horseradish peroxidase (HRP).

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Figure 3: Immunohistochemistry - Anti-PNPLA3 Antibody (A82487)

Negative control for PNPLA3 expression in Human Kidney analyzed by immunohistochemistry. Tissue was paraffin-embedded, and staining procedure was performed in the absence of primary antibody.

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Figure 4: Immunofluorescence - Anti-PNPLA3 Antibody (A82487)

PNPLA3 expression in HepG2 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-PNPLA3 Antibody (A82487) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Mitochondrial staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.