Figure 1: Western Blot - Anti-PNGase Antibody (A91092)
Western blot analysis of extracts of various cell lines, using Anti-PNGase Antibody (A91092) at 1:900 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.
Immunofluorescence analysis of PC-12 cells using Anti-PNGase Antibody (A91092) at a dilution of 1:50 (40x lens). DAPI was used to stain the cell nuclei (blue).
