Figure 1: Western Blot - Anti-PKC alpha (phospho Thr638) Antibody [ARC1591] (A309082)
Western blot analysis of extracts of various cell lines, using Anti-PKC alpha (phospho Thr638) Antibody [ARC1591] (A309082) at 1:1,000 dilution. C2C12 cells were treated by PMA/TPA (200 nM) at 37°C for 30 minutes after serum-starvation overnight. C6 cells were treated by CIP(20uL/400ul) at 37°C for 1 hour. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 10s.
Prodotti alternativi a Anti-PKC alpha (phospho Thr638) Anticorpo [ARC1591] (A309082)