Figure 1: Western Blot - Anti-PKC alpha Antibody [ARC0197] (A13227)
Western blot analysis of extracts of various cell lines, using Anti-PKC alpha Antibody [ARC0197] (A13227) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-PKC alpha Antibody [ARC0197] (A13227) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Figure 3: Western Blot - Anti-PKC alpha Antibody [ARC0197] (A13227)
Immunoprecipitation analysis of 300µg extracts of HeLa cells using 3µg of Anti-PKC alpha Antibody [ARC0197] (A13227). This Western blot was performed on the immunoprecipitate using Anti-PKC alpha Antibody [ARC0197] (A13227) at a dilution of 1:1000.
Prodotti alternativi a Anti-PKC alpha Anticorpo [ARC0197] (A13227)