Anti-PIM2 Antibody (A84957)

Name: Anti-PIM2 Antibody
Brand: Antibodies.com
SKU: A84957
Price: 495.0 USD
Availability: InStock

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$495

Goat polyclonal antibody to PIM2 for ELISA, WB and IHC.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 4-6 business days.

Specifications

Name

Anti-PIM2 Antibody

Description

Goat polyclonal antibody to PIM2.

Applications

ELISA, WB, IHC

Dilutions

ELISA: 1:64,000, WB: 0.5-1µg/ml, IHC: 5µg/ml

Reactivity

Human

Immunogen

Synthetic peptide corresponding to the internal region (near N terminus), amino acids 23-36, of human PIM2.

Sequence

C-KDREAFEAEYRLGP

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Molecular Weight

38 kDa

Product Form

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Pim-2h, Serine/threonine-protein kinase pim-2

Isotype Controls

Goat IgG (A121671)

Suitable Secondaries

See all Anti-Goat IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

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Western Blot - Anti-PIM2 Antibody (A84957)

PIM2 expression in HEK293 cell lysate analyzed by western blot. Cells were transfected to overexpress MYC tagged PIM2 an lysed in RIPA buffer. Primary incubation was performed for 1 hour on 10µg protein per lane with Anti-PIM2 Antibody (A84957) at 0.1µg/ml (Lane A) or Anti-MYC at 1:1000 dilution (Lane C) and detected with chemiluminescence. Control: Mock-transfected HEK293 cells incubated with Anti-PIM2 Antibody (A84957) at 0.1mg/ml (Lane B).

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Immunohistochemistry - Anti-PIM2 Antibody (A84957)

PIM2 expression in Human Heart analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-PIM2 Antibody (A84957) at 5µg/ml and revealed with alkaline phosphatase (AP).

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