Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
DDXBP1, DEAD/H box-binding protein 1, E3 SUMO-protein ligase PIAS1, E3 SUMO-protein transferase PIAS1, GBP, Gu-binding protein, Protein inhibitor of activated STAT protein 1, RNA helicase II-binding protein
Western Blot - Anti-PIAS1 Antibody [ARC1116] (A305639)
Western blot analysis of extracts of various cell lines, using Anti-PIAS1 Antibody [ARC1116] (A305639) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.
Western Blot - Anti-PIAS1 Antibody [ARC1116] (A305639)
Western blot analysis of extracts of Mouse lung, using Anti-PIAS1 Antibody [ARC1116] (A305639) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 3min.
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