Anti-PGC1 alpha Anticorpo (A326276)

Name: Anti-PGC1 alpha Antibody
Brand: Antibodies.com
SKU: A326276
Price: 448.0 USD
Availability: InStock

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$448

Anticorpo policlonale di capra contro PGC1 alpha per ELISA, WB, IF e Flow Cytometry.

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Consegna

Spese di spedizione

$40

Tempi di consegna

3-5 Giorni lavorativi

Contattaci

Telefono

+1 (314) 370-6046

E-mail

orders@antibodies.com

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Descrizione Prodotto

Nome

Anti-PGC1 alpha Antibody

Descrizione

Goat polyclonal antibody to PGC1 alpha.

Applicazioni

ELISA, WB, IF, Flow Cytometry

Diluizioni consigliate

ELISA: 1:128,000, WB: 1.5-3µg/ml, IF: 10µg/ml, Flow Cytometry: 10µg/ml

Reattività

Human

Immunogeno

Synthetic peptide corresponding to the internal region of human PGC1 alpha.

Sequenza

RDSVSPPKSLFSQC

Specie ospite

Goat

Clonalità

Polyclonal

Isotipo

IgG

Coniugare

Unconjugated

Purificazione

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentrazione

500 µg/ml

Peso molecolare

70+90 kDa

MW previsto

91.0kDa

Forma del prodotto

Liquid

Formulazione

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Sinonimi

L PGC 1alpha, LEM6, Ligand effect modulator 6, Peroxisome proliferative activated receptor gamma coactivator 1, Peroxisome proliferative activated receptor gamma coactivator 1 alpha, Peroxisome proliferator activated receptor gamma coactivator 1 alpha, Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B4, Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B4 3ext, Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B4 8a, Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B5, Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B5 NT, Peroxisome proliferator-activated receptor gamma coactivator 1-alpha, PGC 1 (alpha), PGC 1 alpha, PGC 1v, PGC-1-alpha, PGC1, PGC1(alpha), PGC1A, PGC1v, PPAR gamma coactivator 1, PPAR gamma coactivator 1 alpha, PPAR gamma coactivator 1 alpha 3 ligand effect modulator 6, PPAR gamma coactivator variant form, PPAR-gamma coactivator 1-alpha, PPARGC 1 alpha, PPARGC-1-alpha, PPARGC1, PPARGC1A, PRGC1_HUMAN

Controlli isotipo

Goat IgG (A121671)

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (8)

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Figure 1: Western Blot - Anti-PGC1 alpha Antibody (A326276)

PGC1 alpha expression in A431 (A), HepG2 (B), and HEK293 nuclear (C) cell lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary incubation was performed with Anti-PGC1 alpha Antibody (A326276) at 3µg/ml (A-B) or 1.5µg/ml (C) and detected by chemiluminescence.

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Figure 2: Immunofluorescence - Anti-PGC1 alpha Antibody (A326276)

PGC1 alpha expression in A431 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-PGC1 alpha Antibody (A326276) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic and nuclear staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Figure 3: Immunofluorescence - Anti-PGC1 alpha Antibody (A326276)

PGC1 alpha expression in U2OS cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-PGC1 alpha Antibody (A326276) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Nuclear staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Figure 4: Flow Cytometry - Anti-PGC1 alpha Antibody (A326276)

PGC1 alpha expression in A431 cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-PGC1 alpha Antibody (A326276) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.