Anti-PGC1 alpha Antibody (A305531)

Name: Anti-PGC1 alpha Antibody
Brand: Antibodies.com
SKU: A305531

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This product is discontinued

Anti-PGC1 alpha Antibody (A305531) has been discontinued and is no longer available.

View all PGC1 alpha Antibodies.

Specifications

Name

Anti-PGC1 alpha Antibody

Description

Rabbit polyclonal antibody to PGC1 alpha.

Applications

WB, IHC

Dilutions

WB: 1:100-1:500, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 600-700 of human PGC1a (NP_037393.1).

Sequence

CYYYESSHYRHRTHRNSPLYVRSRSRSPYSRRPRYDSYEEYQHERLKREEYRREYEKRESERAKQRERQRQKAIEERRVIYVGKIRPDTTRTELRDRFEVF

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

100 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

LEM6, Ligand effect modulator 6, Peroxisome proliferator-activated receptor gamma coactivator 1-alpha, PGC-1-alpha, PGC1, PGC1A, PPARGC1, PPARGC1A

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (5)

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Western Blot - Anti-PGC1 alpha Antibody (A305531)

Western blot analysis of various lysates, using Anti-PGC1 alpha Antibody (A305531) at 1:400 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Western Blot - Anti-PGC1 alpha Antibody (A305531)

Western blot analysis of extracts from wild type(WT) and PGC1alpha Rabbit polyclonal antibody knockdown (KD) HeLa cells, using Anti-PGC1 alpha Antibody (A305531) at 1:400 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Immunohistochemistry - Anti-PGC1 alpha Antibody (A305531)

Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-PGC1 alpha Antibody (A305531) at a dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-PGC1 alpha Antibody (A305531)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-PGC1 alpha Antibody (A305531) at a dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.