Figure 1: Western Blot - Anti-Perilipin-1 Antibody [ARC1122] (A306256)
Western blot analysis of extracts of various cell lines, using Anti-Perilipin-1 Antibody [ARC1122] (A306256) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3min.
Immunohistochemistry analysis of paraffin-embedded rat brown adipose using Anti-Perilipin-1 Antibody [ARC1122] (A306256) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse brown adipose using Anti-Perilipin-1 Antibody [ARC1122] (A306256) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunofluorescence analysis of rat fat using Anti-Perilipin-1 Antibody [ARC1122] (A306256) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of mouse brown adipose using Anti-Perilipin-1 Antibody [ARC1122] (A306256) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).