Recombinant rabbit monoclonal [PRF1/8662R] antibody to Perforin.
Specificità
Perforin is a pore-forming protein that leads to osmotic lysis of the target cells and subsequently enables granzymes to enter the target cells and activate apoptosis. Perforin has structural and functional similarities to complement component 9 (C9). Like C9, this protein creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. It is one of the main cytolytic proteins of cytolytic granules, and is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Defects in this gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood. The expression of perforin is reportedly upregulated in activated CD8+ T-cells, natural killer cells and some CD4+ T-cells.
Applicazioni
IHC-P
Controllo positivo
HDLM-2 cells. Human spleen.
Diluizioni consigliate
IHC: 1-2 µg/ml
Reattività
Human
Immunogeno
Recombinant human Perforin-1 protein fragment (around Amino Acids 413-552). The exact sequence is proprietary.
Specie ospite
Rabbit
Clonalità
Monoclonal
Clona ID
PRF1/8662R
Isotipo
IgG
Catene leggere
kappa
Coniugare
Unconjugated
Purificazione
Protein A/G chromatography.
Concentrazione
200 µg/ml
Peso molecolare
75 kDa
Forma del prodotto
Liquid
Formulazione
Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.
Conservazione
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.