Anti-PDPK1 (phospho Ser241) Anticorpo (A10962)

Name: Anti-PDPK1 (phospho Ser241) Antibody
Brand: Antibodies.com
SKU: A10962
Price: 435.0 USD
Availability: InStock

Clicca sull'immagine per la visualizzazione estesa

$435

Anticorpo policlonale di coniglio contro PDPK1 (phospho Ser241) per WB e ICC/IF.

Garanzia al 100%

Garanzia di corrispondenza del prezzo

Consegna

Spese di spedizione

$40

Tempi di consegna

5-8 Giorni lavorativi

Contattaci

Telefono

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanzie

Garanzia di qualità al 100%

Garanzia di corrispondenza del prezzo

Descrizione Prodotto

Nome

Anti-PDPK1 (phospho Ser241) Antibody

Descrizione

Rabbit polyclonal antibody to PDPK1 (phospho Ser241).

Applicazioni

WB, ICC/IF

Diluizioni consigliate

WB: 1:500-1:2,000, ICC/IF: 1:50-1:200

Reattività

Human, Mouse, Rat

Immunogeno

A synthetic phosphorylated peptide around S241 of human PDK1/PDPK1 (NP_002604.1).

Sequenza

ANSFV

Specie ospite

Rabbit

Clonalità

Polyclonal

Isotipo

IgG

Coniugare

Unconjugated

Purificazione

Affinity purification.

Peso molecolare

60 - 70 kDa

Forma del prodotto

Liquid

Formulazione

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Sinonimi

3 phosphoinositide dependent protein kinase 1, 3-phosphoinositide-dependent protein kinase 1, hPDK 1, hPDK1, MGC20087, MGC35290, OTTHUMP00000159109, OTTHUMP00000159110, OTTHUMP00000174525, PDK1, PDPK1_HUMAN, PDPK2, PDPK2P, PkB kinase, PkB kinase like gene 1, PkB like 1, PRO0461, Protein kinase

Controlli isotipo

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (4)

Clicca sull'immagine per la visualizzazione estesa

Figure 1: Western Blot - Anti-PDPK1 (phospho Ser241) Antibody (A10962)

Western blot analysis of extracts of NIH/3T3 cells, using Anti-PDPK1 (phospho Ser241) Antibody (A10962) at 1:1,000 dilution. NIH/3T3 cells were treated by PDGF (100 ng/ml) at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 60s.

Clicca sull'immagine per la visualizzazione estesa

Figure 2: Immunofluorescence - Anti-PDPK1 (phospho Ser241) Antibody (A10962)

Immunofluorescence analysis of C6 cells using Anti-PDPK1 (phospho Ser241) Antibody (A10962) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

Clicca sull'immagine per la visualizzazione estesa

Figure 3: Immunofluorescence - Anti-PDPK1 (phospho Ser241) Antibody (A10962)

Immunofluorescence analysis of NIH-3T3 cells using Anti-PDPK1 (phospho Ser241) Antibody (A10962) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

Clicca sull'immagine per la visualizzazione estesa

Figure 4: Immunofluorescence - Anti-PDPK1 (phospho Ser241) Antibody (A10962)

Immunofluorescence analysis of U-2 OS cells using Anti-PDPK1 (phospho Ser241) Antibody (A10962) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).