The antibody detects endogenous levels of PDK1 only when phosphorylated at tyrosine 9.
Applications
IHC
Dilutions
Immunohistochemistry: 1:50~1:100
Reactivity
Human
Immunogen
Peptide sequence around phosphorylation site of tyrosine 9(A-L-Y(p)-D-A) derived from Human PDK1.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunohistochemical analysis of paraffin-embedded human brain tissue using PDK1 (Phospho-Tyr9) antibody #11719 (left)or the same antibody preincubated with blocking peptide (right).
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