Anti-PDHA1 (phospho Ser293) Anticorpo [ARC53489] (A306031)

Name: Anti-PDHA1 (phospho Ser293) Antibody [ARC53489]
Brand: Antibodies.com
SKU: A306031
Price: 540.0 USD
Availability: InStock

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$540

Anticorpo monoclonale [ARC53489] di coniglio contro PDHA1 (phospho Ser293) per WB, ICC/IF e IP.

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Consegna

Spese di spedizione

$40

Tempi di consegna

5-8 Giorni lavorativi

Contattaci

Telefono

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanzie

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Descrizione Prodotto

Nome

Anti-PDHA1 (phospho Ser293) Antibody [ARC53489]

Descrizione

Rabbit monoclonal [ARC53489] antibody to PDHA1 (phospho Ser293).

Applicazioni

WB, ICC/IF, IP

Diluizioni consigliate

WB: 1:2,000-1:20,000, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000

Reattività

Human, Mouse, Rat

Immunogeno

A synthetic phosphorylated peptide around S293 of human PDHA1 (P08559).

Sequenza

GHSMS

Specie ospite

Rabbit

Clonalità

Monoclonal

Clona ID

ARC53489

Isotipo

IgG

Coniugare

Unconjugated

Purificazione

Affinity purification.

Peso molecolare

43 kDa

Forma del prodotto

Liquid

Formulazione

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Sinonimi

mitochondrial, ODPA_HUMAN, PDH, PDHA, PDHCE1A, PDHE1 A type I, PDHE1-A type I, PHE1A, Pyruvate Dehydrogenase (lipoamide) alpha 1, Pyruvate dehydrogenase complex E1 alpha polypeptide 1, Pyruvate dehydrogenase complex, E1 alpha polypeptide 1, Pyruvate Dehydrogenase E1 alpha, Pyruvate dehydrogenase E1 component subunit alpha, Pyruvate dehydrogenase E1 component subunit alpha somatic form mitochondrial, Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial, Pyruvate Dehydrogenase E1-alpha subunit, Pyruvate dehydrogenase, alpha-1, somatic form

Controlli isotipo

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (5)

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Figure 1: Western Blot - Anti-PDHA1 (phospho Ser293) Antibody [ARC53489] (A306031)

Western blot analysis of extracts of various cell lines, using Anti-PDHA1 (phospho Ser293) Antibody [ARC53489] (A306031) at 1:1,000 dilution. Hela cells and NIH/3T3 cells and C6 cells were treated by CIP(20uL/400ul) at 37°C for 1 hour. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 3s.

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Figure 2: Western Blot - Anti-PDHA1 (phospho Ser293) Antibody [ARC53489] (A306031)

Western blot analysis of extracts of HeLa cells, using Anti-PDHA1 (phospho Ser293) Antibody [ARC53489] (A306031) at 1:115000 dilution. 293T cells were treated by Î»-PP mixed solution (1ul) at 30°C for 30 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 3: Western Blot - Anti-PDHA1 (phospho Ser293) Antibody [ARC53489] (A306031)

Western blot analysis of extracts of HeLa cells, using Anti-PDHA1 (phospho Ser293) Antibody [ARC53489] (A306031) at 1:20,000 dilution. NIH/3T3 cells were treated by CIP(20uL/400ul) at 37°C for 1 hour. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 4: Immunofluorescence - Anti-PDHA1 (phospho Ser293) Antibody [ARC53489] (A306031)

Immunofluorescence analysis of NIH/3T3 using Anti-PDHA1 (phospho Ser293) Antibody [ARC53489] (A306031) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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