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Anti-PD-L1 Anticorpo (A80578)

Western Blot - Anti-PD-L1 Antibody (A80578) - Antibodies.com Western Blot - Anti-PD-L1 Antibody (A80578) - Antibodies.com Immunohistochemistry - Anti-PD-L1 Antibody (A80578) - Antibodies.com Immunohistochemistry - Anti-PD-L1 Antibody (A80578) - Antibodies.com
$335
Product Datasheet

Anticorpo policlonale di coniglio contro PD-L1 per WB e IHC.

Garanzia al 100%
Garanzia di corrispondenza del prezzo

Spese di spedizione
$40
Tempi di consegna
5-8 Giorni lavorativi

Telefono
+1 (314) 370-6046
Lun - Ven, 8:00 - 16:00 AST
E-mail
orders@antibodies.com

Nome
Anti-PD-L1 Antibody
Descrizione
Rabbit polyclonal antibody to PD-L1.
Applicazioni
WB, IHC
Diluizioni consigliate
WB: 1:100-1:500, IHC: 1:50-1:200
Reattività
Human, Mouse, Rat
Immunogeno
A synthetic peptide corresponding to a sequence within amino acids 1-50 of human PD-L1/CD274 (NP_054862.1).
Sequenza
MRIFAVFIFMTYWHLLNAFTVTVPKDLYVVEYGSNMTIECKFPVEKQLDL
Specie ospite
Rabbit
Clonalità
Polyclonal
Isotipo
IgG
Coniugare

Unconjugated

Purificazione
Affinity purification.
Peso molecolare
40 - 50 kDa
Forma del prodotto
Liquid
Formulazione
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Conservazione
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Sinonimi
B7 H, B7 H1, B7 homolog 1, B7-H1, B7H, B7H1, CD 274, CD274, CD274 antigen, CD274 molecule, MGC142294, MGC142296, OTTHUMP00000021029, PD L1, PD1L1_HUMAN, PDCD1 ligand 1, PDCD1L1, PDCD1LG1, PDL 1, PDL1, Programmed cell death 1 ligand 1, Programmed death ligand 1, RGD1566211
Controlli isotipo
Secondari adatti
Dichiarazione di non responsabilità
Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.
Western Blot - Anti-PD-L1 Antibody (A80578) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 1: Western Blot - Anti-PD-L1 Antibody (A80578)
Western blot analysis of extracts of various cell lines, using Anti-PD-L1 Antibody (A80578) at 1:500 dilution. A-549 cells were treated by IFNG (100 ng/ml) at 37°C for 48 hours. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
Western Blot - Anti-PD-L1 Antibody (A80578) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 2: Western Blot - Anti-PD-L1 Antibody (A80578)
Western blot analysis of extracts of Mouse skeletal muscle, using Anti-PD-L1 Antibody (A80578) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.
Immunohistochemistry - Anti-PD-L1 Antibody (A80578) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 3: Immunohistochemistry - Anti-PD-L1 Antibody (A80578)
Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using Anti-PD-L1 Antibody (A80578) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-PD-L1 Antibody (A80578) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 4: Immunohistochemistry - Anti-PD-L1 Antibody (A80578)
Immunohistochemistry analysis of paraffin-embedded human placenta using Anti-PD-L1 Antibody (A80578) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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