Anti-PCK2 Anticorpo (A83245)

Name: Anti-PCK2 Antibody
Brand: Antibodies.com
SKU: A83245
Price: 480.0 USD
Availability: InStock

Questo prodotto è identico a Abcam (ab40843). Scopri di più con meno.

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$480

Anticorpo policlonale di capra contro PCK2 per ELISA, WB, IF e Flow Cytometry.

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Consegna

Spese di spedizione

$40

Tempi di consegna

3-5 Giorni lavorativi

Contattaci

Telefono

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanzie

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Descrizione Prodotto

Nome

Anti-PCK2 Antibody

Descrizione

Goat polyclonal antibody to PCK2.

Applicazioni

ELISA, WB, IF, Flow Cytometry

Diluizioni consigliate

ELISA: 1:4,000, WB: 1-3µg/ml, IF: 10µg/ml, Flow Cytometry: 10µg/ml

Reattività

Human, Mouse

Reattività incrociata

This antibody does not cross react with PCK1.

Immunogeno

Synthetic peptide corresponding to the internal region of human PCK2.

Sequenza

KPWKPGDKEPCAH

Specie ospite

Goat

Clonalità

Polyclonal

Isotipo

IgG

Coniugare

Unconjugated

Purificazione

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentrazione

500 µg/ml

Peso molecolare

70 kDa

MW previsto

70.7kDa

Forma del prodotto

Liquid

Formulazione

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Sinonimi

EC 4.1.1.32, GTP mitochondrial precursor, HGNC:8725, mitochondrial, Mitochondrial phosphoenolpyruvate carboxykinase 2, OTTHUMP00000164700, PCKGM_HUMAN, PE, PEP carboxykinase, PEPCK, PEPCK deficiency mitochondrial, PEPCK M, PEPCK-M, PEPCK2, Phosphoenolpyruvate carboxykinase 2 (mitochondrial), Phosphoenolpyruvate carboxykinase 2 mitochondrial, Phosphoenolpyruvate carboxykinase [GTP], Phosphoenolpyruvate carboxylase, Phosphopyruvate carboxylase

Controlli isotipo

Goat IgG (A121671)

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (4)

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Figure 1: Western Blot - Anti-PCK2 Antibody (A83245)

PCK2 expression in A431 (A), HEK293 (B), and HepG2 (C) cell lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary incubation was performed with Anti-PCK2 Antibody (A83245) at 2µg/ml (A-B) or 1µg/ml (C) and detected by chemiluminescence.

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Figure 2: Western Blot - Anti-PCK2 Antibody (A83245)

PCK2 expression in NIH3T3 cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-PCK2 Antibody (A83245) at 2µg/ml and detected by chemiluminescence.

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Figure 3: Immunofluorescence - Anti-PCK2 Antibody (A83245)

PCK2 expression in U2OS cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-PCK2 Antibody (A83245) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Figure 4: Flow Cytometry - Anti-PCK2 Antibody (A83245)

PCK2 expression in MCF7 cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-PCK2 Antibody (A83245) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.