Anti-PBX1 Anticorpo [PCRP-PBX1-3C8] (A277732)

Name: Anti-PBX1 Antibody [PCRP-PBX1-3C8]
Brand: Antibodies.com
SKU: A277732
Price: 525.0 USD
Availability: InStock

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$525

Anticorpo monoclonale [PCRP-PBX1-3C8] di topo contro PBX1 per ELISA, IP, Flow Cytometry e IF.

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Alternative Formats and ConjugatesAlternative Formats

Carrier Free and Preservative Free

Consegna

Spese di spedizione

$40

Tempi di consegna

4-6 Giorni lavorativi

Contattaci

Telefono

+1 (314) 370-6046

E-mail

orders@antibodies.com

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Anticorpo Monospecifico

Descrizione Prodotto

Nome

Anti-PBX1 Antibody [PCRP-PBX1-3C8]

Descrizione

Mouse monoclonal [PCRP-PBX1-3C8] antibody to PBX1.

Specificità

Pbx 1, 2, 3 and 4 are members of the TALE (three amino acid loop extension) family of homeodomain-containing proteins. Human pre-B cell acute leukemias are frequently associated with a t(1;19)(q23;p13.3) chromosomal rearrangement, which creates a chimeric gene encoding a fusion between the E2A and Pbx 1 gene products. Pbx 2 and Pbx 3 share 92% and 94% respective identities with Pbx 1 over a 266 amino acid region flanking their homeobox domains, while all three proteins are quite divergent at their amino- and carboxy-termini. Two forms of Pbx 1 and Pbx 3 each differ primarily in their carboxy-termini and result from alternative mRNA splicing. Unlike other homeotic selector genes which are expressed transiently during development and differentiation, Pbx gene transcripts are ubiquitously expressed in both fetal and adult tissues and cell lines. Additionally, Pbx 2 and Pbx 3 transcripts are detected in lymphoid cells, which do not express Pbx 1. Pbx 4 expression is confined to the testis, especially to spermatocytes in the pachytene stage of the first meiotic prophase.

Applicazioni

ELISA, IP, Flow Cytometry, IF

Diluizioni consigliate

IP: 1-2µg / 100-500µg proteins, Flow Cytometry: 1-2 µg/million cells, IF: 1-2 µg/ml

Reattività

Human

Immunogeno

Recombinant full-length human PBX1 protein.

Specie ospite

Mouse

Clonalità

Monoclonal

Clona ID

PCRP-PBX1-3C8

Isotipo

IgG2b

Coniugare

Unconjugated

Purificazione

Protein A/G chromatography.

Concentrazione

200 µg/ml

Peso molecolare

86.1 kDa

Forma del prodotto

Liquid

Formulazione

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Note generali

This monoclonal antibody is also available in a different formulation without BSA and Sodium Azide - Anti-PBX1 Antibody [PCRP-PBX1-3C8] - BSA and Azide free (A278320).

Sinonimi

DKFZp686B09108, Homeo box protein PBX1, Homeo box protein PRL, Homeobox protein PBX 1, Homeobox protein PBX1, Homeobox protein PRL, MGC126627, PBX 1, PBX1_HUMAN, Pre B cell leukemia homeobox 1, Pre B cell leukemia transcription factor 1, Pre-B-cell leukemia transcription factor 1, PRL

Controlli isotipo

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (4)

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Figure 1: SDS-PAGE - Anti-PBX1 Antibody [PCRP-PBX1-3C8] (A277732)

SDS-PAGE analysis of Anti-PBX1 Antibody [PCRP-PBX1-3C8] under non-reduced and reduced conditions; showing intact IgG and intact heavy and light chains, respectively. SDS-PAGE analysis confirms the integrity and purity of the antibody.

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Figure 2: Flow Cytometry - Anti-PBX1 Antibody [PCRP-PBX1-3C8] (A277732)

Flow cytometric analysis of PFA-fixed HeLa cells using Anti-PBX1 Antibody [PCRP-PBX1-3C8] followed by Goat Anti-Mouse IgG (CF® 488) (Blue). Isotype Control (Red).

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Figure 3: Immunofluorescence - Anti-PBX1 Antibody [PCRP-PBX1-3C8] (A277732)

Immunofluorescent analysis of PFA-fixed HeLa cells stained with Anti-PBX1 Antibody [PCRP-PBX1-3C8] followed by Goat Anti-Mouse IgG (CF® 488) (Green). CF® 640A Phalloidin (Red).

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Figure 4: Anti-PBX1 Antibody [PCRP-PBX1-3C8] (A277732)

Analysis of protein array containing more than 19,000 full-length human proteins using Anti-PBX1 Antibody [PCRP-PBX1-3C8]. Z-Score and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt^TM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt^TM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target; a MAb is considered to be specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.