Anti-PAX5 Antibody [ARC1654] (A305552)

Name: Anti-PAX5 Antibody [ARC1654]
Brand: Antibodies.com
SKU: A305552
Price: 485.0 USD
Availability: InStock

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$485

Rabbit monoclonal [ARC1654] antibody to PAX5 for WB, IHC and IP.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-PAX5 Antibody [ARC1654]

Description

Rabbit monoclonal [ARC1654] antibody to PAX5.

Applications

WB, IHC, IP

Dilutions

WB: 1:100-1:500, IHC: 1:50-1:200, IP: 1:50-1:200

Reactivity

Human

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 200-300 of human PAX5 (Q02548).

Sequence

DEGIQESPVPNGHSLPGRDFLRKQMRGDLFTQQQLEVLDRVFERQHYSDIFTTTEPIKPEQTTEYSAMASLAGGLDDMKANLASPTPADIGSSVPGPQSYP

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1654

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

45 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

B-cell-specific transcription factor, BSAP, Paired box protein Pax-5

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

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Western Blot - Anti-PAX5 Antibody [ARC1654] (A305552)

Western blot analysis of extracts of Raji cells, using Anti-PAX5 Antibody [ARC1654] (A305552) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Immunohistochemistry - Anti-PAX5 Antibody [ARC1654] (A305552)

Immunohistochemistry analysis of paraffin-embedded human appendix tissue using Anti-PAX5 Antibody [ARC1654] (A305552) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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