Rabbit polyclonal antibody to p70 S6 Kinase (Phospho-Thr389 / 412)
Specificity
The antibody detects endogenous level of p70 S6 Kinase only when phosphorylated at tyrosine 389 / tyrosine 412.
Applications
WB
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of threonine389 / 412(G-F-T(p)-Y-V) derived from Human p70 S6 Kinase .
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Western blot analysis of extracts from Jurkat cells treated with Insulin using Phospho-p70 S6 Kinase (Thr389/412) antibody #11974.The lane on the right is treated with the antigen-specific peptide.
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