Western blot analysis of extracts of various cell lines, using Anti-p53 Antibody (A11620) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Western blot analysis of extracts of NIH/3T3 cells, using Anti-p53 Antibody (A11620) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry analysis of paraffin-embedded rat liver using Anti-p53 Antibody (A11620) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-p53 Antibody (A11620) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunoprecipitation analysis of 200µg extracts of U-251MG cells using 3µg of Anti-p53 Antibody (A11620). This Western blot was performed on the immunoprecipitate using Anti-p53 Antibody (A11620) at a dilition of 1:1000.
![Immunohistochemistry - Anti-p53 Antibody [rBP53-12] (A250176) - Antibodies.com](https://cdn.antibodies.com/image/catalog/250/A250176_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-p53 Antibody [TP53/2092R] (A250192) - Antibodies.com](https://cdn.antibodies.com/image/catalog/250/A250192_1.jpg?profile=product_alternative)
