Western blot analysis of extracts of various cell lines, using Anti-P4HA2 Antibody (A14543) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Western blot analysis of extracts of Rat placenta, using Anti-P4HA2 Antibody (A14543) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.
Immunofluorescence analysis of NIH/3T3 cells using Anti-P4HA2 Antibody (A14543) at a dilution of 1:50 (40x lens). DAPI was used to stain the cell nuclei (blue).
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