Samples: Whole cell lysate (50 µg) from MCF-7, RT-4, A2058, A-431, and BJ cells prepared using NETN lysis buffer. Antibody: Anti-p40 Antibody [BC28] (A300662) was used at 1:1000. Secondary: HRP-conjugated goat anti-mouse IgG. Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.