Immunocytochemistry/Immunofluorescence analysis of human cervical cancer cell line (HeLa), fixed in 2% formaldehyde for 20 minutes at room temperature, using Anti-p38 alpha / MAPK14 Antibody (A305111), at 1:100 for 12 hours at 4°C. The secondary antibody used was FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at room temperature. Localization: Mitochondrion. Cytoplasm. Nucleus. Magnification: 20x.(A) DAPI (blue) nuclear stain. (B) Anti-p38 Antibody. (C) Composite.
Figure 2: Western Blot - Anti-p38 alpha / MAPK14 Antibody (A305111)
Western blot analysis of human cancer cell lines showing detection of p38 protein using Anti-p38 alpha / MAPK14 Antibody (A305111) at 1:4,000 for 2 hours at room temperature. Load: 15 µgprotein. Block: 1.5% BSA for 30 minutes at room temperature. The secondary antibody used was Donkey Anti-Rabbit IgG: HRP for 1 hour at room temperature.
Immunocytochemistry/Immunofluorescence analysis of human cervical cancer cell line (HeLa), fixed in 2% formaldehyde for 20 minutes at room temperature, using Anti-p38 alpha / MAPK14 Antibody (A305111), at 1:100 for 12 hours at 4°C. The secondary antibody used was FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at room temperature. Localization: Mitochondrion. Cytoplasm. Nucleus. Magnification: 100x.(A) DAPI (blue) nuclear stain. (B) Anti-p38 Antibody. (C) Composite.
