Anti-p21 Antibody (A307747)

Name: Anti-p21 Antibody
Brand: Antibodies.com
SKU: A307747

Enlarge Image

This product is discontinued

Anti-p21 Antibody (A307747) has been discontinued and is no longer available.

View all p21 Antibodies.

Specifications

Name

Anti-p21 Antibody

Description

Rabbit polyclonal antibody to p21.

Applications

WB, IHC, IP

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, IP: 1:100-1:500

Reactivity

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-164 of human CDKN1A/p21CIP1 (NP_000380.1).

Sequence

MSEPAGDVRQNPCGSKACRRLFGPVDSEQLSRDCDALMAGCIQEARERWNFDFVTETPLEGDFAWERVRGLGLPKLYLPTGPRRGRDELGGGRRPGTSPALLQGTAEEDHVDLSLSCTLVPRSGEQAEGSPGGPGDSQGRKRRQTSMTDFYHSKRRLIFSKRKP

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

CAP20, CDK-interacting protein 1, CDKN1, CDKN1A, CIP1, Cyclin-dependent kinase inhibitor 1, MDA-6, MDA6, Melanoma differentiation-associated protein 6, PIC1, SDI1, WAF1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

Enlarge Image

Western Blot - Anti-p21 Antibody (A307747)

Western blot analysis of extracts of MCF7 cells, using Anti-p21 Antibody (A307747) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

Enlarge Image

Immunohistochemistry - Anti-p21 Antibody (A307747)

Immunohistochemistry analysis of paraffin-embedded human liver using Anti-p21 Antibody (A307747) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Enlarge Image

Western Blot - Anti-p21 Antibody (A307747)

Immunoprecipitation analysis of 300µg extracts of MCF7 cells using 3µg of Anti-p21 Antibody (A307747). This Western blot was performed on the immunoprecipitate using Anti-p21 Antibody (A307747) at a dilution of 1:500.