This kit relies on the presence of nicks in the DNA which can be identified by TdT, an enzyme that catalyzes the addition of dUTPs that are labeled with fluorescein. This kit provides all the essential components with an optimized assay protocol, suitable for fluorescence microplate reader, fluorescence microscope, or flow cytometer. Its signal can be easily detected at the popular FITC channel ( Ex/Em = 490nm/520 nm).
Tipo di campione
Cells and tissues
Componenti
TdT Enzyme, Equilibration Buffer (5X), Label Mix Green, DAPI (500X), BSA Working Solution, TritonX-100 (100%), DNase I (5 U/µl)
Assorbimento massimo
490 nm
Massimi di emissione
520 nm
Conservazione
Shipped at 4°C. Store at -20°C. Once opened, refer to the Protocol Manual for storage conditions of individual components. Do not use past expiration date!
Dichiarazione di non responsabilità
Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.
