Anti-Nucleostemin Antibody (A9877)

Name: Anti-Nucleostemin Antibody
Brand: Antibodies.com
SKU: A9877
Price: 355.0 USD
Availability: InStock

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$355

Rabbit polyclonal antibody to Nucleostemin for WB, ICC/IF and IP.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-Nucleostemin Antibody

Description

Rabbit polyclonal antibody to Nucleostemin.

Applications

WB, ICC/IF, IP

Dilutions

WB: 1:500-1:2,000, ICC/IF: 1:10-1:100, IP: 1:50-1:100

Reactivity

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human GNL3 (NP_996562.1).

Sequence

MTCHKRYKIQKKVREHHRKLRKEAKKRGHKKPRKDPGVPNSAPFKEALLREAELRKQRLEELKQQQKLDRQKELEKKRKLETNPDIKPSNVEPMEKEFGLCKTENKAKSGKQNSKKLYCQELKKVIEASDVVLEVLDARDPLGCRCPQVEEAIVQSGQKKLVLILNKSDLVPKENLESWLNYLKKELPTVVFRASTKPKDKGKITKRVKAKKNAAPFRSEVCFGKEGLWKLLGGFQETCS

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

62 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

E2-induced gene 3 protein, E2IG3, GNL3, Guanine nucleotide-binding protein-like 3, NNP47, Novel nucleolar protein 47, NS, Nucleolar GTP-binding protein 3

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-Nucleostemin Antibody (A9877)

Western blot analysis of extracts of 293T cells, using Anti-Nucleostemin Antibody (A9877) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Immunofluorescence - Anti-Nucleostemin Antibody (A9877)

Immunofluorescence analysis of MCF7 cells using Anti-Nucleostemin Antibody (A9877). DAPI was used to stain the cell nuclei (blue).

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Western Blot - Anti-Nucleostemin Antibody (A9877)

Immunoprecipitation analysis of 200µg extracts of 293T cells using 3µg of Anti-Nucleostemin Antibody (A9877). This Western blot was performed on the immunoprecipitate using Anti-Nucleostemin Antibody (A9877) at a dilution of 1:500.

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