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Anti-Nucleophosmin Anticorpo (A307817)

Nome
Anti-Nucleophosmin Antibody
Descrizione
Rabbit polyclonal antibody to Nucleophosmin.
Applicazioni
WB, IHC, ICC/IF
Diluizioni consigliate
WB: 1:500-1:1,000, IHC: 1:100-1:500, ICC/IF: 1:50-1:200
Reattività
Human, Mouse, Rat
Immunogeno
Recombinant fusion protein containing a sequence corresponding to amino acids 9-122 of human NPM1 (NP_002511.1).
Sequenza
MSPLRPQNYLFGCELKADKDYHFKVDNDENEHQLSLRTVSLGAGAKDELHIVEAEAMNYEGSPIKVTLATLKMSVQPTVSLGGFEITPPVVLRLKCGSGPVHISGQHLVAVEED
Specie ospite
Rabbit
Clonalità
Polyclonal
Isotipo
IgG
Coniugare

Unconjugated

Purificazione
Affinity purification.
Peso molecolare
38 kDa
Forma del prodotto
Liquid
Formulazione
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.
Conservazione
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Sinonimi
B23, MGC104254, NO38, NPM, NPM1, NPM_HUMAN, Nucleolar phosphoprotein B23, Nucleolar protein NO38, Nucleophosmin (nucleolar phosphoprotein B23 numatrin), nucleophosmin nucleoplasmin family member 1, Nucleophosmin/nucleoplasmin family member 1, Numatrin, OTTHUMP00000161024, OTTHUMP00000161025, OTTHUMP00000223397, OTTHUMP00000223398
Controlli isotipo
Secondari adatti
Dichiarazione di non responsabilità
Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.
Western Blot - Anti-Nucleophosmin Antibody (A307817) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 1: Western Blot - Anti-Nucleophosmin Antibody (A307817)
Western blot analysis of extracts of various cell lines, using Anti-Nucleophosmin Antibody (A307817) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.
Western Blot - Anti-Nucleophosmin Antibody (A307817) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 2: Western Blot - Anti-Nucleophosmin Antibody (A307817)
Western blot analysis of extracts of various cell lines, using Anti-Nucleophosmin Antibody (A307817) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry - Anti-Nucleophosmin Antibody (A307817) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 3: Immunohistochemistry - Anti-Nucleophosmin Antibody (A307817)
Immunohistochemistry analysis of paraffin-embedded human liver using Anti-Nucleophosmin Antibody (A307817) at a dilution of 1:500 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-Nucleophosmin Antibody (A307817) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 4: Immunohistochemistry - Anti-Nucleophosmin Antibody (A307817)
Immunohistochemistry analysis of paraffin-embedded mouse spinal cord using Anti-Nucleophosmin Antibody (A307817) at a dilution of 1:500 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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