Contattaci
+1 (314) 370-6046 oContattaci
Visit our English Website
Antibodies.com LogoAntibodies.com Logo
Accedi / RegistratiIl Mio Account
0

Nuclear Factor 1 Cell Based Kit Elisa (A102823)

Nome
Nuclear Factor 1 Cell Based ELISA Kit
Tipo di kit
Cell-based (qualitative)
Principio di kit
This Nuclear Factor 1 Cell Based ELISA Kit allows for the detection of Nuclear Factor 1 and the effects that certain stimulation conditions have on Nuclear Factor 1 expression in different cell lines. Qualitative determination of Nuclear Factor 1 concentration is achieved by an indirect ELISA format. In essence, the Nuclear Factor 1 is captured by Anti-Nuclear Factor 1 Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this Nuclear Factor 1 Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.
Piattaforma
Microplate
Tipo di rilevamento
Colorimetric
Tipo di campione
Adherent cells and suspension cells.
Campo di rilevamento
> 5000 Cells
Tempo del kit
4 hours 30 minutes
Reattività
Human, Mouse, Rat
Precision
Intra-assay CV: < 8%, Inter-assay CV: < 10%
Conservazione
Store at + 4°C. Stable for 6 months.
Sinonimi
1110019L22Rik, 1500041O16Rik, AA589446, AI746521, CAAT box transcription factor, CCAAT binding transcription factor, CCAAT box binding transcription factor, CCAAT-box-binding transcription factor, CNFI C, CTF, CTF5, MGC137374, MGC20153, NF I, NF I/C, NF-I/C, NF1 C, NF1-C, NF1C, NFI, NFI-C, NFI/C, NFIC, NFIC_HUMAN, Nuclear factor 1 C type, Nuclear factor 1 C-type, Nuclear factor 1/C, Nuclear factor I/C, Nuclear factor I/C (CCAAT binding transcription factor), TGGCA binding protein, TGGCA-binding protein, Transcription factor NFIC
Dichiarazione di non responsabilità
Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.
Western Blot - Nuclear Factor 1 Cell Based ELISA Kit (CB5975) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 1: Western Blot - Nuclear Factor 1 Cell Based ELISA Kit (CB5975)
Western blot analysis of extracts from rat brain/HuvEc cells, using Anti-Nuclear Factor 1 Antibody. The lane on the right is treated with the synthesized peptide.
Protocol Diagram - Nuclear Factor 1 Cell Based ELISA Kit (CB5975) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 2: Nuclear Factor 1 Cell Based ELISA Kit (CB5975)
A diagram showing the protocol for Nuclear Factor 1 Cell Based ELISA Kit.
Protocol Summary - Nuclear Factor 1 Cell Based ELISA Kit (CB5975) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 3: Nuclear Factor 1 Cell Based ELISA Kit (CB5975)
A summary of the protocol for Nuclear Factor 1 Cell Based ELISA Kit.
Western Blot - Control Antibody - Nuclear Factor 1 Cell Based ELISA Kit (CB5975) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 4: Western Blot - Nuclear Factor 1 Cell Based ELISA Kit (CB5975)
The mouse monoclonal antibody to GAPDH is used as a positive control inNuclear Factor 1 Cell Based ELISA Kit. Anti-GAPDH Antibody was tested for specificity by western blot with tissue lysates from human, mouse, and rat.
Menù Principale Contattaci 0Check-out
Inizio pagina