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Anti-NSUN5 Anticorpo (A11908)

Nome
Anti-NSUN5 Antibody
Descrizione
Rabbit polyclonal antibody to NSUN5.
Applicazioni
WB, IHC
Diluizioni consigliate
WB: 1:500-1:1,000, IHC: 1:50-1:200
Reattività
Human, Mouse, Rat
Immunogeno
Recombinant fusion protein containing a sequence corresponding to amino acids 127-429 of human NSUN5 (NP_060514.1).
Sequenza
RPGPASQLPRFVRVNTLKTCSDDVVDYFKRQGFSYQGRASSLDDLRALKGKHFLLDPLMPELLVFPAQTDLHEHPLYRAGHLILQDRASCLPAMLLDPPPGSHVIDACAAPGNKTSHLAALLKNQGKIFAFDLDAKRLASMATLLARAGVSCCELAEEDFLAVSPSDPRYHEVHYILLDPSCSGSGMPSRQLEEPGAGTPSPVRLHALAGFQQRALCHALTFPSLQRLVYSTCSLCQEENEDVVRDALQQNPGAFRLAPALPAWPHRGLSTFPGAEHCLRASPETTLSSGFFVAVIERVEVPR
Specie ospite
Rabbit
Clonalità
Polyclonal
Isotipo
IgG
Coniugare

Unconjugated

Purificazione
Affinity purification.
Peso molecolare
47 kDa
Forma del prodotto
Liquid
Formulazione
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Conservazione
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Sinonimi
FLJ10267, MGC986, NOL1, NOL1-related protein, NOL1/NOP2/Sun domain family member 5, NOL1R, NOP2/Sun domain family, member 5, NOP2/Sun domain family, member 5A, NSUN5_HUMAN, p120, Putative methyltransferase NSUN5, WBSCR20, WBSCR20A, Williams Beuren syndrome chromosome region 20A, Williams-Beuren syndrome chromosomal region 20A protein, Williams-Beuren syndrome critical region protein 20 copy A
Controlli isotipo
Secondari adatti
Dichiarazione di non responsabilità
Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.
Western Blot - Anti-NSUN5 Antibody (A11908) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 1: Western Blot - Anti-NSUN5 Antibody (A11908)
Western blot analysis of extracts of Rat brain, using Anti-NSUN5 Antibody (A11908) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Western Blot - Anti-NSUN5 Antibody (A11908) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 2: Western Blot - Anti-NSUN5 Antibody (A11908)
Western blot analysis of extracts of various cell lines, using Anti-NSUN5 Antibody (A11908) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
Immunohistochemistry - Anti-NSUN5 Antibody (A11908) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 3: Immunohistochemistry - Anti-NSUN5 Antibody (A11908)
Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-NSUN5 Antibody (A11908)Immunohistochemistry analysis of paraffin-embedded Mouse kidney using NSUN5 antibody ( A5992) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-NSUN5 Antibody (A11908) - Antibodies.comClicca sull'immagine per la visualizzazione estesa
Figure 4: Immunohistochemistry - Anti-NSUN5 Antibody (A11908)
Immunohistochemistry analysis of paraffin-embedded mouse spinal cord using Anti-NSUN5 Antibody (A11908)Immunohistochemistry analysis of paraffin-embedded Mouse spinal cord using NSUN5 antibody ( A5992) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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