Anti-NQO1 Anticorpo (A82564)

Name: Anti-NQO1 Antibody
Brand: Antibodies.com
SKU: A82564
Price: 480.0 USD
Availability: InStock

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$480

Anticorpo policlonale di capra contro NQO1 per ELISA, WB, IF e IHC.

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Consegna

Spese di spedizione

$40

Tempi di consegna

3-5 Giorni lavorativi

Contattaci

Telefono

+1 (314) 370-6046

E-mail

orders@antibodies.com

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Descrizione Prodotto

Nome

Anti-NQO1 Antibody

Descrizione

Goat polyclonal antibody to NQO1.

Applicazioni

ELISA, WB, IF, IHC

Diluizioni consigliate

ELISA: 1:32,000, WB: 0.3-1µg/ml, IHC: 3.75µg/ml, IF: 5µg/ml

Reattività

Human

Immunogeno

Synthetic peptide corresponding to the internal region of human NQO1 (Isoform a).

Sequenza

C-DKGPFRSKKAVLS

Specie ospite

Goat

Clonalità

Polyclonal

Isotipo

IgG

Coniugare

Unconjugated

Purificazione

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentrazione

500 µg/ml

Peso molecolare

30 kDa

MW previsto

30.9kDa

Forma del prodotto

Liquid

Formulazione

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Sinonimi

Azoreductase, Cytochrome b 5 reductase, DHQU, DIA 4, DIA4, Diaphorase (NADH/NADPH), Diaphorase (NADH/NADPH) (cytochrome b 5 reductase), Diaphorase 4, Dioxin inducible 1, DT diaphorase, DT-diaphorase, DTD, Menadione reductase, NAD(P)H dehydrogenase quinone 1, NAD(P)H dehydrogenase [quinone] 1, NAD(P)H menadione oxidoreductase 1 dioxin inducible, NAD(P)H quinone dehydrogenase 1, NAD(P)H: menadione oxidoreductase 1 dioxin inducible 1, NAD(P)H:menadione oxidoreductase 1, NAD(P)H:Quinone acceptor oxidoreductase type 1, NAD(P)H:quinone oxidoreductase 1, NAD(P)H:quinone oxireductase, NMOR 1, NMOR I, NMOR1, NMORI, NQO 1, NQO1_HUMAN, Phylloquinone reductase, QR 1, QR1, Quinone reductase 1

Controlli isotipo

Goat IgG (A121671)

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (3)

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Figure 1: Western Blot - Anti-NQO1 Antibody (A82564)

NQO1 expression in Human Kidney lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-NQO1 Antibody (A82564) at 0.3µg/ml and detected by chemiluminescence.

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Figure 2: Immunofluorescence - Anti-NQO1 Antibody (A82564)

NQO1 expression in HepG2 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-NQO1 Antibody (A82564) at 5µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 5µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Figure 3: Immunohistochemistry - Anti-NQO1 Antibody (A82564)

NQO1 expression in Human Small Intestine analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-NQO1 Antibody (A82564) at 3.75µg/ml and revealed with alkaline phosphatase (AP).

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