Anti-NKCC1 Anticorpo (A84750)

Name: Anti-NKCC1 Antibody
Brand: Antibodies.com
SKU: A84750
Price: 480.0 USD
Availability: InStock

Questo prodotto è identico a Abcam (ab99558). Scopri di più con meno.

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$480

Anticorpo policlonale di capra contro NKCC1 per ELISA, IHC, IF e Flow Cytometry.

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Consegna

Spese di spedizione

$40

Tempi di consegna

3-5 Giorni lavorativi

Contattaci

Telefono

+1 (314) 370-6046

E-mail

orders@antibodies.com

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Descrizione Prodotto

Nome

Anti-NKCC1 Antibody

Descrizione

Goat polyclonal antibody to NKCC1.

Applicazioni

ELISA, IHC, IF, Flow Cytometry

Diluizioni consigliate

ELISA: 1:64,000, IHC: 3.75µg/ml, IF: 10µg/ml, Flow Cytometry: 10µg/ml

Reattività

Human

Immunogeno

Synthetic peptide corresponding to the internal region of human NKCC1.

Sequenza

C-NALQHSIRLSGVEDH

Specie ospite

Goat

Clonalità

Polyclonal

Isotipo

IgG

Coniugare

Unconjugated

Purificazione

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentrazione

500 µg/ml

Forma del prodotto

Liquid

Formulazione

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Sinonimi

Basolateral Na-K-Cl symporter, BSC, BSC2, Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1, mBSC2, MGC104233, PPP1R141, Protein phosphatase 1, regulatory subunit 141, S12A2_HUMAN, Slc12a2, sodium-potassium-chloride cotransporter 1, Solute carrier family 12 (sodium/potassium/chloride transporter), member 2, solute carrier family 12 (sodium/potassium/chloride transporters) member 2, Solute carrier family 12 member 2, sy-ns

Controlli isotipo

Goat IgG (A121671)

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (3)

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Figure 1: Immunofluorescence - Anti-NKCC1 Antibody (A84750)

NKCC1 expression in HeLa cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-NKCC1 Antibody (A84750) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic/vesicle and membrane staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Figure 2: Flow Cytometry - Anti-NKCC1 Antibody (A84750)

NKCC1 expression in HeLa cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-NKCC1 Antibody (A84750) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.

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Figure 3: Immunohistochemistry - Anti-NKCC1 Antibody (A84750)

NKCC1 expression in Human Colon analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-NKCC1 Antibody (A84750) at 3.75µg/ml and revealed with alkaline phosphatase (AP).