Rabbit polyclonal antibody to NFkB-p65 (Phospho-Thr254)
Specificità
The antibody detects endogenous level of NF-kB p65 only when phosphorylated at threonine 254.
Applicazioni
WB, IHC, IF
Reattività
Human, Mouse, Rat
Immunogeno
Peptide sequence around phosphorylation site of threonine 254 (F-R-T(p)-P-P) derived from Human NFkB-p65.
Specie ospite
Rabbit
Clonalità
Polyclonal
Coniugare
Unconjugated
Purificazione
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentrazione
1 mg/ml
Formulazione
Supplied at 1.0mg / mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Conservazione
Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NFkB-p65 (Phospho-Thr254) Antibody #11010 (left) or the same antibody preincubated with blocking peptide (right).
