Rabbit polyclonal antibody to NFkB-p65 (Phospho-Ser529)
Specificità
The antibody detects endogenous level of NFkB-p65 only when phosphorylated at serine 529.
Applicazioni
WB, IHC, IF
Diluizioni consigliate
Western blotting: 1:500~1:1000 Immunohistochemistry: 1:50~1:100 Immunofluorescence: 1:100~1:200
Reattività
Human
Immunogeno
Peptide sequence around phosphorylation site of serine 529 (L-L-S(p)-G-D) derived from Human NFkB-p65.
Specie ospite
Rabbit
Clonalità
Polyclonal
Coniugare
Unconjugated
Purificazione
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentrazione
1 mg/ml
MW previsto
65kd
Formulazione
Supplied at 1.0mg / mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Conservazione
Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NFkB-p65 (Phospho-Ser529) Antibody #11217 (left) or the same antibody preincubated with blocking peptide (right).
Western blot analysis of extracts from MCF cells, treated with TNFa+CA or calf intestinal phosphatase (CIP), using NFκB-p65 (Phospho-Ser529) Antibody #11217.
