Anti-NFIL3 Antibody (A83792)

Name: Anti-NFIL3 Antibody
Brand: Antibodies.com
SKU: A83792
Price: 495.0 USD
Availability: InStock

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$495

Goat polyclonal antibody to NFIL3 for ELISA, WB and Flow Cytometry.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 4-6 business days.

Specifications

Name

Anti-NFIL3 Antibody

Description

Goat polyclonal antibody to NFIL3.

Applications

ELISA, WB, Flow Cytometry

Dilutions

ELISA: 1:2,000, WB: 0.5-2µg/ml, Flow Cytometry: 10µg/ml

Reactivity

Human

Immunogen

Synthetic peptide corresponding to the C terminal region of human NFIL3.

Sequence

C-RLIATQPISASDSG

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Molecular Weight

60 kDa

Predicted MW

51.5kDa

Product Form

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

E4 promoter-binding protein 4, E4BP4, IL3BP1, Interleukin-3 promoter transcriptional activator, Interleukin-3-binding protein 1, Nuclear factor interleukin-3-regulated protein, Transcriptional activator NF-IL3A

Isotype Controls

Goat IgG (A121671)

Suitable Secondaries

See all Anti-Goat IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

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Western Blot - Anti-NFIL3 Antibody (A83792)

NFIL3 expression in Jurkat nuclear cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 20µg protein was run per lane. Primary antibody was performed with Anti-NFIL3 Antibody (A83792) at 1µg/ml and detected by chemiluminescence.

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Flow Cytometry - Anti-NFIL3 Antibody (A83792)

NFIL3 expression in Human peripheral blood monocytes (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-NFIL3 Antibody (A83792) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.

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