Anti-NF-kappaB p65 (phospho Thr435) Antibody (A93732)

Name: Anti-NF-kappaB p65 (phospho Thr435) Antibody
Brand: Antibodies.com
SKU: A93732
Price: 190.0 USD
Availability: InStock

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$190

Rabbit polyclonal antibody to NF-kappaB p65 (phospho Thr435) for WB, IHC, IP and ELISA.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 6-8 business days.

Specifications

Name

Anti-NF-kappaB p65 (phospho Thr435) Antibody

Description

Rabbit polyclonal antibody to NF-kappaB p65 (phospho Thr435).

Specificity

This antibody detects endogenous levels of NF-kappaB p65 only when phosphorylated at Thr435.

Applications

WB, IHC, IP, ELISA

Dilutions

WB: 1:500-1:1000, IHC: 1:50-1:100, IF: 1:100-1:500, ELISA: 1:20000

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide derived from human NF-kappaB p65 around the phosphorylation site of Thr435 (amino acids 402-451).

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.

Molecular Weight

60kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

NFKB3, Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, RELA, Transcription factor p65

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

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Immunohistochemistry - Anti-NF-kappaB p65 (phospho Thr435) Antibody (A93732)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Anti-NF-kappaB p65 (phospho Thr435) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.

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Western Blot - Anti-NF-kappaB p65 (phospho Thr435) Antibody (A93732)

Western blot analysis of lysates from COS7 cells treated with TNF-alpha using Anti-NF-kappaB p65 (phospho Thr435) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.

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