Anti-Neutrophil Elastase Antibody (A89123)

Name: Anti-Neutrophil Elastase Antibody
Brand: Antibodies.com
SKU: A89123
Price: 345.0 USD
Availability: InStock

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$345

Rabbit polyclonal antibody to Neutrophil Elastase for WB, IHC and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Specifications

Name

Anti-Neutrophil Elastase Antibody

Description

Rabbit polyclonal antibody to Neutrophil Elastase.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 168-267 of human Neutrophil Elastase (ELANE) (NP_001963.1).

Sequence

VLQELNVTVVTSLCRRSNVCTLVRGRQAGVCFGDSGSPLVCNGLIHGIASFVRGGCASGLYPDAFAPVAQFVNWIDSIIQRSEDNPCPHPRDPDPASRTH

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

30 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Bone marrow serine protease, ELA2, ELANE, Elastase-2, HLE, Human leukocyte elastase, Medullasin, PMN elastase

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (7)

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Western Blot - Anti-Neutrophil Elastase Antibody (A89123)

Western blot analysis of various lysates, using Anti-Neutrophil Elastase Antibody (A89123) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Immunohistochemistry - Anti-Neutrophil Elastase Antibody (A89123)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-Neutrophil Elastase Antibody (A89123) at a dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-Neutrophil Elastase Antibody (A89123)

Immunohistochemistry analysis of paraffin-embedded human esophagus using Anti-Neutrophil Elastase Antibody (A89123) at a dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-Neutrophil Elastase Antibody (A89123)

Immunohistochemistry analysis of paraffin-embedded rat bone marrow using Anti-Neutrophil Elastase Antibody (A89123) at a dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.