Anti-Neuroligin 1 Antibody [S97A-31] (A304803)

Name: Anti-Neuroligin 1 Antibody [S97A-31]
Brand: Antibodies.com
SKU: A304803
Price: 520.0 USD
Availability: InStock

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$520

Mouse monoclonal [S97A-31] antibody to Neuroligin 1 for WB, IHC and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Tags:

Synaptic Marker

Specifications

Name

Anti-Neuroligin 1 Antibody [S97A-31]

Description

Mouse monoclonal [S97A-31] antibody to Neuroligin 1.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:1,000, ICC/IF: 1:100

Reactivity

Human, Mouse, Rat

Cross Reactivity

This antibody does not cross-react with other Neuroligins.

Immunogen

Fusion protein amino acids 718-843 (cytoplasmic C-terminus) of rat Neuroligin-1. Mouse: 99% identity (125/126 amino acids identical). Human: 99% identity (125/126 amino acids identical) >40% identity with Neuroligin-2 and -3..

Host

Mouse

Clonality

Monoclonal

Clone ID

S97A-31

Isotype

IgG1

Conjugate

Unconjugated

Purification

Protein G purification.

Concentration

1 mg/ml

Molecular Weight

~120 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.1% Sodium Azide

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

KIAA1070, Neuroligin-1, NLGN1

Isotype Controls

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Immunofluorescence - Anti-Neuroligin 1 Antibody [S97A-31] (A304803)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cells (SH-SY5Y), fixed in 4% PFA for 15 min, using Anti-Neuroligin 1 Antibody [S97A-31] (A304803), at 1:100 for overnight at 4°C with slow rocking. The secondary antibody used was AlexaFluor 488 at 1:1,000 for 1 hour at room temperature. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 minutes at room temperature.(A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Neuroligin 1 Antibody (D) Composite.

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Western Blot - Anti-Neuroligin 1 Antibody [S97A-31] (A304803)

Western blot analysis of mouse brain membrane showing detection of ~120 kDa Neuroligin 1 protein using Anti-Neuroligin 1 Antibody [S97A-31] (A304803) at 1:200 for 16 hours at 4°C. Lane 1: Molecular Weight Ladder. Lane 2: mouse brain Membrane. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Mouse IgG: HRP at 1:1,000 for 1 hour room temperature. Color Development: ECL solution for 6 min in room temperature. Predicted/Observed Size: ~120 kDa.

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Immunofluorescence - Anti-Neuroligin 1 Antibody [S97A-31] (A304803)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cell line (SK-N-BE, fixed in 4% formaldehyde for 15 min at room temperature, using Anti-Neuroligin 1 Antibody [S97A-31] (A304803), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Mouse ATTO 488 at 1:100 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5,000 for 60min room temperature, 5min room temperature. Localization: Cell Membrane, Nucleus. Magnification: 60X.(A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Neuroligin 1 Antibody. (D) Composite.

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