Anti-NeuN Antibody [NeuN/7071R] - BSA and Azide free
Descrizione
Recombinant rabbit monoclonal [NeuN/7071R] antibody to NeuN.
Specificità
NeuN antibody specifically recognizes the DNA-binding, neuron-specific protein NeuN, which is present in most CNS and PNS neuronal cell types of all vertebrates tested. NeuN protein distributions are apparently restricted to neuronal nuclei and some proximal neuronal processes in both fetal and adult brain although, some neurons fail to be recognized by NeuN at all ages: INL retinal cells, Cajal-Retzius cells, Purkinje cells, inferior olivary and dentate nucleus neurons, and sympathetic ganglion cells are examples. Immunohistochemically detectable NeuN protein first appears at developmental timepoints that correspond with the withdrawal of the neuron from the cell cycle and/or with the initiation of terminal differentiation of the neuro. Immunoreactivity appears around E9.5 in the mouse neural tube and is extensive throughout the developing nervous system by E12.5. Strong nuclear staining suggests a nuclear regulatory protein function; however, no evidence currently exists as to whether the NeuN protein antigen has a function in the distal cytoplasm or whether it is merely synthesized there before being transported back into the nucleus. No difference between protein isolated from purified nuclei and whole brain extract on immunoblots has been found.
Applicazioni
IHC-P
Diluizioni consigliate
IHC-P: 1-2 µg/ml
Reattività
Human
Immunogeno
Synthetic peptide, within amino acids 30-60, of human NeuN protein. The exact sequence is proprietary.
Specie ospite
Rabbit
Clonalità
Monoclonal
Clona ID
NeuN/7071R
Isotipo
IgG
Catene leggere
kappa
Coniugare
Unconjugated
Purificazione
Protein A/G chromatography.
Concentrazione
1 mg/ml
Peso molecolare
38 kDa
Forma del prodotto
Liquid
Formulazione
Supplied in 10mM Phosphate Buffered Saline; without Sodium Azide and carrier free.
Conservazione
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
SDS-PAGE analysis of Anti-NeuN Antibody [NeuN/7071R] under non-reduced and reduced conditions; showing intact IgG and intact heavy and light chains, respectively. SDS-PAGE analysis confirms the integrity and purity of the antibody.
Prodotti alternativi a Anti-NeuN Anticorpo [NeuN/7071R] (A278555)